Distinct metabonomic signatures of Polygoni Multiflori Radix Praeparata against glucolipid metabolic disorders
Distinct metabonomic signatures of Polygoni Multiflori Radix Praeparata against glucolipid metabolic disorders
复制标题
制何首乌对抗糖脂代谢紊乱的独特代谢组学特征
DOI：
10.1093/jpp/rgab012
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发表时间：
2021-03-18
影响因子：
3.3
通讯作者：
Yang, Xing-Xin
中科院分区：
文献类型：
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作者：
Yang, Ya-Qin;Meng, Fan-Ying;Yang, Xing-Xin
目标葡萄糖代谢性疾病（GLMD）促进了一系列主要的慢性疾病。 Polygoni Multilori radix Preparata（PMRP）在预防和治疗GLMD方面已被广泛认可。我们先前报道说，PMRP的水提取物及其主要的生物活性成分，例如多糖（POL）和2,3,5,4'-二氢 - 二羟基-Stilbene-2-o-Beta-beta-d-d-葡萄糖苷（TSG），可以减轻GLMD。线粒体可以减轻我们的GLMD，PMRP和TSG的POL仍然未知。在这项研究中，我们阐明了我们，POL和TSG对使用线粒体级别质量替代工具来调节线粒体功能障碍和减轻GLMD的影响。通过高糖和高脂（HS-HF）饮食建立了GLMD的大鼠模型。在胃部内，将大鼠，POL和TSG持续12周。通过超高的液相色谱/质谱法分析肝线粒体代谢产物，然后进行多元统计分析，以识别差异代谢物和代谢途径。关键发现WE，POL，POL，POL和TSG可以显着恢复Liver Mitchondririririation river Mitochoncondriria tormal tormal tormal tormal tormal tormal tormal tornal of Forman torm tormal torman torm tormal tornal tormant。在分别从WE，GOL和TSG组获得的肝线粒体样品中，总共确定了16，7和14个差异代谢产物。发现这些代谢产物主要参与磷脂，组氨酸，丙氨酸，天冬氨酸，谷氨酸代谢和精氨酸生物合成。结论PMRP可以通过调节线粒体的代谢plmd pmrp来改善肝线粒体功能，以质地质地质量glmd。因此，PMRP的应用可能是一种有前途的线粒体调节剂/营养素来减轻GLMD相关疾病，线粒体替代学可能会提供对评估药物作用机制的见解。
Objectives Glucolipid metabolic disorders (GLMD) promote a series of major chronic diseases. Polygoni Multilori Radix Preparata (PMRP) has been widely acknowledged in the prevention and treatment of GLMD. We previously reported that water extract (WE) of PMRP and its major bioactive constituents such as polysaccharides (POL) and 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glucoside (TSG) could alleviate GLMD.The mitochondrial dysfunction is an important mechanism of GLMD, but the underlying mechanisms behind the regulation of mitochondria to alleviate GLMD by WE, POL from PMRP and TSG are still unknown.Methods In this study, we elucidated the effects of WE, POL, andTSG towards regulating the mitochondria' dysfunction and alleviating GLMD using mitochondrial metabonomics. A rat model of GLMD was established by high-sugar and high-fat (HS-HF) diet. Rats were intragastrically given WE, POL, and TSG for 12 weeks. The liver mitochondrial metabolites were analyzed by ultra-high-performance liquid chromatography/mass spectrometry followed by multivariate statistical analysis to identify the differential metabolites and metabolic pathways.Key findingsThe WE, POL, andTSG could significantly restore the level of endogenous metabolites in liver mitochondria toward normal status. In total, sixteen, seven, and fourteen differential metabolites were identified in the liver mitochondrial samples obtained from the WE, GOL, andTSG groups, respectively. These metabolites were found to be mainly involved in glycerol phospholipid, histidine, alanine, aspartic acid, glutamate metabolism, and arginine biosynthesis.Conclusions PMRP could improve the liver mitochondrial function by regulating the mitochondria' metabolic pathways to alleviate GLMD. Therefore, the application of PMRP might be a promising mitochondrial regulator/nutrient for alleviating GLMD-associated diseases and the mitochondrial metabonomics might provide insights into the evaluation of the efficacies and mechanisms of action of drugs.