INDUCTION OF SPREADING DURING FIBROBLAST MOVEMENT
INDUCTION OF SPREADING DURING FIBROBLAST MOVEMENT
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DOI：
10.1083/jcb.81.3.684
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发表时间：
1979-01-01
影响因子：
7.8
通讯作者：
CHEN, WT
中科院分区：
文献类型：
--
作者：
CHEN, WT
本文报道了鸡胚心脏成纤维细胞在培养过程中牵拉引起的伸展现象。细胞铺展的可测量标准（铺展薄层面积和细胞总铺展面积的增加）随着细胞边缘的一部分的收缩而可预测地变化。活动增加。扩散的成纤维细胞的前板层通常缓慢地前进，平均面积增加约0.5。在后缘处收缩开始后的8秒内，展开增加10至30倍，然后稍微降低，使得到1分钟时展开增加5至10倍。在此期间，在前缘处的面积增加与在后缘处的面积减小之间存在线性关系。在接下来的10-15分钟内，扩散逐渐减少至正常。虽然成纤维细胞在不同运动阶段的面积扩展和面积收缩之间的关系不是显著线性的，但它是高度相关的。成纤维细胞扩散的速率可能与细胞作为一个整体的扩散程度呈负相关。
The phenomenon of retraction-induced spreading of embryonic chick heart fibroblasts moving in culture is described. Measurable criteria of cell spreading (increase in area of the spreading lamella and total spread area of the cell) change predictably with retraction of a portion of the cell margin. Ruffling activity increased. The leading lamella of a spread fibroblast ordinarily advances slowly, with an average area increase of .apprx. 21 .mu.m2/min. A 10- to 30-fold increase in spreading occurs within 8 s after onset of retraction at the trailing edge and then decreases slightly so that by 1 min the increase in spreading is 5- to 10-fold. During this period, there is a linear relationship between area increase at the leading edge and area decrease at the trailing edge. During the next 10-15 min, spreading gradually decreases to normal. Although the relationship between area spreading and area retracting of fibroblasts at different phases of movement is not significantly linear, it is highly correlated. The rate of fibroblast spreading may be inversely related to the degree of spreading of the cell as a whole.