CDX1 protein expression in normal, metaplastic, and neoplastic human alimentary tract epithelium
CDX1 protein expression in normal, metaplastic, and neoplastic human alimentary tract epithelium
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DOI：
10.1053/gast.1997.v113.pm9247467
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发表时间：
1997-08-01
期刊：
影响因子：
29.4
通讯作者：
Traber, PG
中科院分区：
文献类型：
--
作者：
Silberg, DG;Furth, EE;Traber, PG
背景和目标：CDX 1是一种在肠发育早期表达的肠上皮特异性转录因子，可能参与调节肠上皮细胞的增殖和分化。我们检测了CDX 1蛋白在化生和肿瘤组织中的表达模式，以了解其在异常分化中的可能作用。方法：组织样本进行染色免疫组化使用亲和纯化，多克隆抗体对CDX 1的肽表位。结果：小肠和结肠上皮细胞核呈特异性染色。食管和胃不表达CDX 1蛋白;然而，肠化生组织的邻近区域强烈表达CDX 1，胃和食管的腺癌具有CDX 1的阳性和阴性核染色，腺瘤性息肉和腺癌中的结肠上皮细胞与同一标本中的正常结肠隐窝相比，染色强度降低。CDX 1可能是重要的从正常的胃和食管上皮细胞的过渡到膀胱型化生。CDX 1在胃和食管腺癌中表达的变异性提示在这些癌的发展中存在不止一种途径。CDX 1在结肠腺癌中的减少可能表明CDX 1在生长调节和维持分化表型中的作用。
Background & Aims: CDX1 is an intestine-specific transcription factor expressed early in intestinal develop ment that may be involved in regulation of proliferation and differentiation of intestinal epithelial cells, We examined the pattern of CDX1 protein expression in metaplastic and neoplastic tissue to provide insight into its possible role in abnormal differentiation. Methods: Tissue samples were stained by immunohistochemistry using an affinity-purified, polyclonal antibody against a peptide epitope of CDX1. Results: Specific nuclear staining was found in epithelial cells of the small intestine and colon. Esophagus and stomach did not express CDX1 protein; however, adjacent areas of intestinal metaplastic tissue intensely stained for CDX1, Adenocarcinomas of the stomach and esophagus had both positive and negative nuclear staining for CDX1, Colonic epithelial cells in adenomatous polyps and adenocarcinomas had a decreased intensity of staining compared with normal colonic crypts in the same specimen, Conclusions: CDX1 may be important in the transition from normal gastric and esophageal epithelium to intestinal-type metaplasia. The variability in expression of CDX1 in gastric and esophageal adenocarcinomas suggests more than one pathway in the develop ment of these carcinomas. The decrease of CDX1 in colonic adenocarcinomas may indicate a role for CDX1 in growth regulation and in the maintenance of the differentiated phenotype.