Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars
Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars
复制标题
三个新 S 等位基因的表征和 S 等位基因特异性 PCR 系统的开发，用于快速鉴定苹果品种中的 S 基因型。
DOI：
10.1007/s11295-009-0237-6
复制
发表时间：
2010-02-01
影响因子：
2.4
通讯作者：
Li, Tianzhong
中科院分区：
文献类型：
--
作者：
Long, Shenshan;Li, Maofu;Li, Tianzhong
苹果（Malus domestica Borkh）是蔷薇科植物中的一员，其配子体自交不亲和性由多态s等位基因控制。鉴定苹果品种的s基因型，可以为苹果品种的正确配种提供依据，对苹果果实的高效生产具有重要意义。本研究鉴定了苹果中3个新的S等位基因，分别命名为S（44）、S（45）和S(46)，并建立了一种利用等位基因特异性聚合酶链反应快速鉴定S基因型的高效分析方法。针对不同的等位基因，选择性设计了19条等位基因特异性引物。利用该方法鉴定了157个苹果品种的s基因型。
Apple (Malus domestica Borkh), a member of the Rosaceae, shows gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. Identifying the S-genotypes of apple cultivars can be applied on correct assignment of apple cultivars to cross-compatibility groups, which is important for the efficient production of apple fruit. This study characterized three new S-alleles (designated S (44) , S (45) , and S (46) ) in apple and developed an efficient analysis method that can be used to characterize S-genotypes by utilizing allele-specific polymerase chain reaction rapidly. Nineteen allele-specific primers were selectively designed to identify different alleles. Using this method, S-genotypes of 157 apple cultivars were identified.