Fast protein folding kinetics.
Fast protein folding kinetics.
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DOI：
10.1017/s003358351400002x
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发表时间：
2014-05
影响因子：
6.1
通讯作者：
Gruebele, Martin
中科院分区：
文献类型：
--
作者：
Gelman, Hannah;Gruebele, Martin
快速折叠蛋白质一直是计算和实验研究的主要焦点，因为它们对两种技术都是可行的：它们足够小，足够快，可以用当前的计算能力进行合理的模拟，但其动力学速度足够慢，可以用专门开发的实验技术进行观察。这种对快速折叠蛋白质的耦合研究提供了一些机制的见解，这些机制允许一些蛋白质在不到1毫秒的时间内找到它们的天然构象，并揭示了理论预测的现象，如下坡折叠。对快速文件夹的研究也告诉我们对“慢”折叠过程的理解：快速文件夹是小的，相对简单的蛋白质结构域，控制它们折叠的原则也控制着更复杂系统的折叠。本文综述了研究快速折叠蛋白的主要理论和实验技术，并对快速折叠研究的主要成果进行了综述。最后，我们研究了从研究快速文件夹中出现的主题，并简要总结了它们在蛋白质折叠中的应用，以及一些需要做的工作。
Fast folding proteins have been a major focus of computational and experimental study because they are accessible to both techniques: they are small and fast enough to be reasonably simulated with current computational power, but have dynamics slow enough to be observed with specially developed experimental techniques. This coupled study of fast folding proteins has provided insight into the mechanisms which allow some proteins to find their native conformation well less than 1 ms and has uncovered examples of theoretically predicted phenomena such as downhill folding. The study of fast folders also informs our understanding of even “slow” folding processes: fast folders are small, relatively simple protein domains and the principles that govern their folding also govern the folding of more complex systems. This review summarizes the major theoretical and experimental techniques used to study fast folding proteins and provides an overview of the major findings of fast folding research. Finally, we examine the themes that have emerged from studying fast folders and briefly summarize their application to protein folding in general as well as some work that is left to do.