Proton NMR spectroscopy of glucose consumption by cultured lens epithelial cells.
Proton NMR spectroscopy of glucose consumption by cultured lens epithelial cells.
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培养晶状体上皮细胞葡萄糖消耗的质子核磁共振波谱。
DOI：
10.1089/jop.1986.2.319
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Lam,DM
中科院分区：
文献类型：
--
作者：
Cheng,HM;Aguiar,E;Ford,JJ;Kelleher,P;Lam,DM
对收集自培养的兔眼透镜上皮细胞的培养基进行质子NMR光谱分析，所述培养的兔眼晶状体上皮细胞与含有5.5mMC-葡萄糖（在C-1位置标记）的Krebs-Ringer溶液一起孵育。比较C-3碳（12 C和13 C）的乳酸-甲基的质子共振强度使我们能够量化己糖单磷酸分流（HMPS）活性。我们的结果表明，上皮细胞在乳酸产生和HMPS活性方面保持稳定至少8小时。此外，虽然tBHP（1 mM）导致通过HMPS的葡萄糖流量增加，但乳酸产生速率不受影响。相比之下，KCN（2 mM）引起乳酸产量增加72%，通过HMPS的葡萄糖消耗轻微（6%）减少。
Proton NMR spectroscopy was performed on media collected from cultured lens epithelial cells of the rabbit eye incubated with Krebs-Ringer's solution containing 5.5mMC-glucose (labeled at the C-1 position). Comparing proton resonance intensities of the lactate-methyl group of the C-3 carbon (both12C and13C) enabled us to quantify the hexose monophosphate shunt (HMPS) activity. Our results showed that the epithelial cells remained stable in both lactate production and HMPS activity for at least 8 hours. In addition, although tBHP (1mM) resulted in an increase of glucose flow through the HMPS, the rate of lactate production was not affected. In contrast, KCN (2mM) caused a 72% increase of lactate production and a slight (6%) decrease of glucose consumption through the HMPS.