Studies on the oligosaccharide sequence of ribonuclease B.
Studies on the oligosaccharide sequence of ribonuclease B.
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核糖核酸酶B寡糖序列的研究。
DOI：
10.1016/s0021-9258(18)62897-2
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
F. Maley
中科院分区：
文献类型：
--
作者：
A. Tarentino;T. Plummer;F. Maley
α-甘露糖苷酶处理来自核糖核酸酶B的天冬酰胺酰低聚糖衍生物，释放出5当量的甘露糖，并产生含有比例为1：2：1的天冬酰胺、N-乙酰葡糖胺和甘露糖的产物。剩余的甘露糖残基对α-甘露糖苷酶的水解具有抗性，但可以通过Smith降解去除，该方法不影响N-乙酰葡糖胺残基。所得产物与由Spinola和Jeanloz化学制备的2-乙酰氨基-4-O-（2-乙酰氨基-2-脱氧-β-d-吡喃葡萄糖基）-N-（4-l-天冬氨酰基）-2-脱氧-β-d-吡喃葡萄糖胺进行相同的层析（J. Biol. Chem.，245，4158（1970））。此外，合成的和分离的化合物与β-N-乙酰氨基葡萄糖苷酶和糖基天冬酰胺酶的反应相似，证明天冬酰胺酰三糖中的甘露糖残基与末端非还原性N-乙酰氨基葡萄糖的3-O位相连，N-乙酰氨基葡萄糖形成与二-N-乙酰壳二糖相同的二糖。
α-Mannosidase treatment of the asparaginyl oligosaccharide derivative from ribonuclease B released 5 eq of mannose and yielded a product containing asparagine,N-acetylglucosamine, and mannose in a ratio of 1:2:1. The remaining mannose residue was resistant to hydrolysis by α-mannosidase, but could be removed by a Smith degradation, a procedure which did not affect theN-acetylglucosamine residues. The resultant product chromatographed identically with 2-acetamido-4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-N-(4-l-aspartyl)-2-deoxy-β-d-glucopyranosylamine prepared chemically by Spinola and Jeanloz (J. Biol. Chem., 245, 4158 (1970)). In addition, both synthetic and isolated compounds reacted similarly on treatment with β-N-acetylglucosaminidase and glycosyl asparaginase.Evidence is presented showing the mannose residue in the asparaginyl trisaccharide to be linked to the 3-Oposition of the terminal nonreducingN-acetylglucosamine and that theN-acetylglucosamines form a disaccharide identical with di-N-acetylchitobiose.