Immune responses of orange-spotted grouper, Epinephelus coioides, against virus-like particles of betanodavirus produced in Escherichia coli
Immune responses of orange-spotted grouper, Epinephelus coioides, against virus-like particles of betanodavirus produced in Escherichia coli
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DOI：
10.1016/j.vetimm.2013.10.003
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发表时间：
2014-01-15
影响因子：
1.8
通讯作者：
Xie, Jun-Feng
中科院分区：
文献类型：
--
作者：
Lai, Yu-Xiong;Jin, Bao-Lei;Xie, Jun-Feng
病毒性神经坏死（viral nervous necrosis，VNN）是一种严重危害海洋养殖鱼类的疾病。病毒样颗粒（VLP）是一种很好的新型疫苗候选人，以控制这种疾病。到目前为止，betanodavirus疫苗研究主要集中在病毒攻击后的体液免疫应答和死亡率。然而，人们对疫苗激活细胞免疫和先天免疫基因知之甚少。在本研究中，橙斑石斑鱼神经坏死病毒（OGNNV）的VLP在原核生物中产生，其进入亚洲鲈鱼细胞的能力与天然病毒相同，表明它们具有类似的结构，OGNNV。然后通过以不同浓度（1.5或15 μ g/g（-1）鱼体重，FBW）和免疫频率（给药一次、两次和三次）肌内接种斜带石斑鱼（Epinealus coioides）来测定VLP的免疫原性。单次接种1.5 μ g g（-1）FBW的剂量足以激发高滴度抗体（平均比阴性对照高3倍），并且早在免疫后1周就具有强中和抗体滴度。此外，定量PCR分析显示，11个与体液，细胞和先天免疫相关的基因在免疫后12 h的肝，脾和头肾上调，与早期抗体反应。总之，我们证明了VLP疫苗接种诱导体液免疫应答，并激活了与针对橙斑石斑鱼β病毒感染的细胞免疫和先天免疫相关的基因。(C)2013爱思唯尔有限公司版权所有。
Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a serious disease of cultured marine fish worldwide. Virus-like particles (VLPs) are one of the good novel vaccine candidates to control this disease. Until now, betanodavirus vaccine studies mainly focused on the humoral immune response and mortality after virus challenge. However, little is known about the activation of genes responsible for cellular and innate immunity by vaccines. In the present study, VLPs of orange-spotted grouper nervous necrosis virus (OGNNV) were produced in prokaryotes and their ability to enter Asian sea bass cells was the same as native virus, suggesting that they possess a similar structure to OGNNV. VLPs immunogenicity was then determined by intramuscularly vaccinating Epinephelus coioides at different concentrations (1.5 or 15 mu g g(-1) fish body weight, FBW) and immunizing frequencies (administration once, twice and thrice). A single vaccination with the dosage of 1.5 mu g g(-1) FBW is enough to provoke high titer antibodies (average 3 fold higher than that of negative control) with strong neutralizing antibody titer as early as 1 week post immunization. Furthermore, quantitative PCR analysis revealed that eleven genes associated with humoral, cellular and innate immunities were up-regulated in the liver, spleen and head kidney at 12 h post immunization, correlating with the early antibody response. In conclusion, we demonstrated that VLP vaccination induced humoral immune responses and activated genes associated with cellular and innate immunity against betanodavirus infection in orange-spotted grouper. (C) 2013 Elsevier B.V. All rights reserved.