LTR sequence and phylogenetic analyses of a newly discovered variant of HTLV-I isolated from the Hagahai of Papua New Guinea.
LTR sequence and phylogenetic analyses of a newly discovered variant of HTLV-I isolated from the Hagahai of Papua New Guinea.
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从巴布亚新几内亚哈加海分离出的新发现的 HTLV-I 变体的 LTR 序列和系统发育分析。
DOI：
10.1016/0042-6822(92)90675-f
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Poiesz,BJ
中科院分区：
文献类型：
--
作者：
Saksena,NK;Sherman,MP;Yanagihara,R;Dube,DK;Poiesz,BJ
对从巴布亚新几内亚最近接触过的一个遥远群体（Hagahai）的健康成员中分离出的人T细胞淋巴瘤/白血病病毒I型（HTLV-1）变体的长末端重复序列（LTR）的631-bp区域进行测序，并与灵长类T细胞淋巴瘤病毒组（PTLV）的其他成员的LTR序列进行比较，包括HTLV-1，猴T细胞淋巴瘤病毒（STLV-I）和HTLV-II。该新几内亚分离株命名为HTLV-I（PNG-1），其LTR的序列分析表明，与原型日本HTLV-I（ATK）和其他HTLV-I和STLV-I分离株的序列差异为8.4%至10.4%，与HTLV-II的差异为48.6%。在转录增强子区域的核心元件、TATA盒启动子以及多聚腺苷酸化信号和位点中发现了很少的突变。此外，观察到的变化并没有显着改变推断的稳定性的雷克斯响应元件，茎环结构的多聚腺苷酸化和雷克斯蛋白结合的关键。基于LTR序列的Dendograms表明，进化成HTLV-I（PNG-1）的病毒株在遥远的过去与其他PTLV分离，就在亚洲的STLV-I祖先之后，但在非洲的STLV-I祖先之前。相比之下，其他HTLV-I分离株被发现代表最近分化的病毒株，并主要根据其地理来源进行聚类。这些数据证实，HTLV-I（PNG-1）是PTLV组的一个新的和独特的变体。此外，我们的分析表明，HTLV-I和STLV-I都可能起源于印度-马来地区，最终传播到非洲，然后传播到新大陆和日本，人类和非人类灵长类动物之间的水平传播可能发生在数千年前。
A 631-bp region of the long terminal repeat (LTR) of a variant of human T-cell lymphoma/leukemia virus type I (HTLV-I), isolated from a healthy member of a remote, recently contacted group (Hagahai) in Papua New Guinea, was sequenced and compared to LTR sequences of other members of the primate T-cell lymphoma virus group (PTLV), including HTLV-I, simian T-cell lymphoma virus (STLV-I) and HTLV-II. Sequence analysis of the LTR of this New Guinean isolate, designated as HTLV-I(PNG-1), indicated a sequence divergence of 8.4% to 10.4% from prototype Japanese HTLV-I(ATK) and other HTLV-I and STLV-I isolates and 48.6% diversity from HTLV-II. Few mutations were found in the core elements of the transcriptional enhancer regions, the TATA box promoter, and the polyadenylation signal and site. Further, the observed changes did not significantly alter the inferred stability of the Rex response element, a stem loop structure critical for polyadenylation and Rex protein binding. Dendograms based on LTR sequences indicated that the strain of virus that evolved into HTLV-I(PNG-1) diverged from the other PTLV in the distant past, just after the progenitors of STLV-I from Asia, but before the ancestors of STLV-I from Africa. By contrast, other HTLV-I isolates were found to represent strains of virus that have diverged more recently and clustered primarily according to their geographical origin. These data confirm that HTLV-I(PNG-1) is a new and distinct variant of the PTLV group. Also, our analyses suggest that both HTLV-I and STLV-I may have originated in the Indo-Malay region and eventually spread to Africa and then to the New World and Japan with horizontal transmission between man and nonhuman primates possibly occurring over thousands of years.