Lignin peroxidases can also oxidize manganese.
Lignin peroxidases can also oxidize manganese.
复制标题
木质素过氧化物酶也可以氧化锰。
DOI：
10.1021/bi00023a025
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Aust,SD
中科院分区：
文献类型：
--
作者：
Khindaria,A;Barr,DP;Aust,SD
材料和方法过氧化氢购自Sigma Chemical Co.(圣路易斯，密苏里州)。超氧化物歧化酶、维生素A和硫酸锰购自奥尔德里奇化学公司(密尔沃基，威斯康星州)。草酸钠、琥珀酸和琥珀酸钠购自Mallinckrodt(巴黎，肯塔基州)。所有的化学品都是试剂级的，并作为购买使用。用纯水(Bamstead NANOpure II系统，比电阻18.0MQ cm-1)制备琥珀酸纳缓冲液。用于产生木质素过氧化物酶的培养条件及其纯化和活性测定如前所述(Tuisel等人，1990年)。整个研究过程中使用的是纯木质素过氧化物酶同工酶H2(pi 4.4)。用分析等电聚焦技术测定LiPH2的纯度。等电聚焦在5.5%的聚丙烯酰胺凝胶中进行，p/梯度为3-10(Bio-Lyte 3-10，BioRad)。该凝胶在使用前立即进行了预聚焦。每口井最多使用15pg的蛋白质，凝胶为
MATERIALS AND METHODSHydrogen peroxide was purchased from Sigma Chemical Co.(St. Louis, MO). Superoxide dismutase, VA, and manganese sulfate were purchased from Aldrich Chemical Co.(Milwaukee, WI). Sodium oxalate, succinic acid and sodium succinate were purchased from Mallinckrodt (Paris, KY). All chemicals were reagent grade and were used as purchased. Sodium succinate buffer was prepared using purified water (Bamstead NANOpure II system; specific resistance 18.0 MQ cm-1).Enzyme Production and Purification. The culture condi-tions used to produce lignin peroxidases and theirpurification and activity assay were as described previously (Tuisel et al., 1990). Pure lignin peroxidase isozyme H2 (pi 4.4) was used throughout this study. The purity of LiPH2 was determined by the analytical isoelectric focusing technique. Isoelectric focusing was done in a 5.5% polyacrylamide gel using a p/gradient of 3—10 (Bio-Lyte 3— 10, BioRad). The gel was prefocused immediately prior to use. A maximum of 15 pg of protein was applied per well, and the gel was