Role of nitric oxide in restenosis after experimental balloon angioplasty in the hypercholesterolemic rabbit: effects on neointimal hyperplasia and vascular remodeling.
Role of nitric oxide in restenosis after experimental balloon angioplasty in the hypercholesterolemic rabbit: effects on neointimal hyperplasia and vascular remodeling.
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DOI：
10.1016/s0735-1097(98)00621-4
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发表时间：
1999-03
影响因子：
24
通讯作者：
T. Tourneau;E. V. Belle;D. Corseaux;Benoit Vallet;Gilles Lebuffe;Bernard Dupuis;J. Lablanche;E. McFadden;Christophe Bauters;Michel E. Bertrand
中科院分区：
文献类型：
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作者：
T. Tourneau;E. V. Belle;D. Corseaux;Benoit Vallet;Gilles Lebuffe;Bernard Dupuis;J. Lablanche;E. McFadden;Christophe Bauters;Michel E. Bertrand
本研究的目的是评估L-精氨酸和NG-硝基-L-精氨酸甲酯（L-NAME）对高胆固醇血症兔球囊血管成形术后新生内膜增生和血管重构的影响。一氧化氮抑制新生内膜增生，但其对血管重塑的影响是unknoweds. METHODS六周后，诱导双侧髂动脉粥样硬化，48只兔子进行了成功的血管成形术在75支血管。8只家兔（急性组）在血管成形术后立即处死。其余动物在血管成形术后接受安慰剂（慢性对照组）或补充有L-精氨酸（1.5 g/kg/天）或L-NAME（15 mg/kg/天）的饮食4周。1.35± 0.62 mm2）。L-精氨酸组内膜面积的增加较低（1.79 ± 0.61 mm 2），而L-NAME组的增加较大（3.23 ± 0.92 mm 2）。与急性组相比，对照组的内弹性膜（IEL）范围明显增加（从2.52 ± 0.66至3.33 ± 0.85 mm 2）; L-NAME组增加更明显（3.90 ± 0.85 mm 2）。相比之下，L-精氨酸组的IEL面积无变化（2.41 ± 0.62 mm 2）。结果表明，慢性组4周后管腔面积无显著差异（对照组：0.74 ± 0.38 mm 2; L-精氨酸：0.50 ± 0.43 mm 2; L-NAME：0.48 ± 0.42 mm 2）。结论：我们的结果表明，L-精氨酸抑制而L-NAME刺激高胆固醇血症兔实验性球囊血管成形术后的新生内膜增生。然而，L-精氨酸组中缺乏血管扩张导致L-NAME和L-精氨酸组中的最终管腔尺寸相似。
OBJECTIVESThe purpose of this study was to assess the effects of L-arginine and NG-nitro-L-arginine methyl ester (L-NAME) on neointimal hyperplasia and vascular remodeling after balloon angioplasty in the hypercholesterolemic rabbit.BACKGROUNDRestenosis after balloon angioplasty is a consequence of both neointimal hyperplasia and vessel remodeling. Nitric oxide inhibits neointimal hyperplasia, but its effect on vessel remodeling is unknown.METHODSSix weeks after induction of bilateral iliac atherosclerosis, 48 rabbits underwent successful angioplasty in 75 vessels. Eight rabbits (acute group) were sacrificed immediately after angioplasty. The remaining animals received either placebo (chronic control group), or a diet supplemented with either L-arginine (1.5 g/kg/day), or L-NAME (15 mg/kg/day) for 4 weeks after angioplasty.RESULTSThe intimal area was significantly greater in the chronic control group compared to the acute group (2.60 ± 1.03 mm2vs. 1.35 ± 0.62 mm2). This increase in intimal area was lower in the L-arginine group (1.79 ± 0.61 mm2), and greater in the L-NAME group (3.23 ± 0.92 mm2). The area circumscribed by the internal elastic lamina (IEL) increased significantly in the control group compared to the acute group (from 2.52 ± 0.66 to 3.33 ± 0.85 mm2); a more marked increase occurred in the L-NAME group (3.90 ± 0.85 mm2). By contrast, IEL area was unchanged in the L-arginine group (2.41 ± 0.62 mm2). As a result, there was no significant difference in lumen area after 4 weeks in the chronic groups (control: 0.74 ± 0.38 mm2; L-arginine: 0.50 ± 0.43 mm2; L-NAME: 0.48 ± 0.42 mm2).CONCLUSIONSOur results demonstrate that L-arginine inhibits whereas L-NAME stimulates neointimal hyperplasia after experimental balloon angioplasty in the hypercholesterolemic rabbit. However, the lack of vessel enlargement in the L-arginine group resulted in a similar final lumen size in the L-NAME and L-arginine groups.