Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines
Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung cancer cell lines
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DOI：
10.3892/ijo.2013.1969
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发表时间：
2013-08-01
影响因子：
5.2
通讯作者：
Chopin, Lisa K.
中科院分区：
文献类型：
--
作者：
Whiteside, Eliza J.;Seim, Inge;Chopin, Lisa K.
参与非小细胞肺癌肿瘤发生的分子机制在很大程度上是未知的;然而，最近的研究表明，长链非编码RNA（lncRNA）可能发挥作用。在这项研究中，我们使用公共数据库，以确定一个mRNA样的，候选的长非编码RNA，GHSROS（GHSR相反链），从生长激素受体基因，生长激素促分泌素受体（GHSR）的反义链转录。实时定量RT-PCR显示GHSROS在肺癌组织中的表达高于邻近的非肿瘤肺组织。与许多长的非编码RNA一样，GHSROS是5'加帽的和3'聚腺苷酸化的（mRNA样），缺乏广泛的开放阅读框架并含有转座因子。GHSROS的工程过表达刺激A549和NCI-H1299非小细胞肺癌细胞系中的细胞迁移，但抑制Beas-2B正常肺衍生的支气管上皮细胞系中的细胞迁移。这表明GHSROS功能可能依赖于致癌背景。GHSROS在肺癌中表达并刺激肺癌细胞系中的细胞迁移，其鉴定有助于越来越多的非编码RNA在肿瘤发生和转移性癌症进展的调节中发挥作用。
The molecular mechanisms involved in non-small cell lung cancer tumourigenesis are largely unknown; however, recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a role. In this study, we used public databases to identify an mRNA-like, candidate long non-coding RNA, GHSROS (GHSR opposite strand), transcribed from the antisense strand of the ghrelin receptor gene, growth hormone secretagogue receptor (GHSR). Quantitative real-time RT-PCR revealed higher expression of GHSROS in lung cancer tissue compared to adjacent, non-tumour lung tissue. In common with many long non-coding RNAs, GHSROS is 5' capped and 3' polyadenylated (mRNA-like), lacks an extensive open reading frame and harbours a transposable element. Engineered overexpression of GHSROS stimulated cell migration in the A549 and NCI-H1299 non-small cell lung cancer cell lines, but suppressed cell migration in the Beas-2B normal lung-derived bronchoepithelial cell line. This suggests that GHSROS function may be dependent on the oncogenic context. The identification of GHSROS, which is expressed in lung cancer and stimulates cell migration in lung cancer cell lines, contributes to the growing number of non-coding RNAs that play a role in the regulation of tumourigenesis and metastatic cancer progression.