DETECTION AND QUANTITATION OF HEPATITIS-B SURFACE-ANTIGEN IMMUNE-COMPLEXES (HBSAG-ICS) BY AN ANTIGEN-SPECIFIC METHOD .2. CIRCULATING IMMUNE-COMPLEXES (CICS) IN PATIENTS WITH HEPATITIS-B AND ASYMPTOMATIC HBSAG-CARRIERS
DETECTION AND QUANTITATION OF HEPATITIS-B SURFACE-ANTIGEN IMMUNE-COMPLEXES (HBSAG-ICS) BY AN ANTIGEN-SPECIFIC METHOD .2. CIRCULATING IMMUNE-COMPLEXES (CICS) IN PATIENTS WITH HEPATITIS-B AND ASYMPTOMATIC HBSAG-CARRIERS
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DOI：
10.1016/0022-1759(80)90257-4
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
NOVAK, E
中科院分区：
文献类型：
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作者：
ANHTUAN, N;NOVAK, E
使用 PEG[聚乙二醇]-胰蛋白酶消化法，在 70% 的急性乙型肝炎患者、5% 的无症状 HBsAg 携带者以及 2 例慢性乙型肝炎病毒感染者中均检测到 HBsAg-CIC（乙型肝炎表面抗原循环免疫复合物）。在一系列检查中，77%的急性乙型肝炎患者的复合HBsAg水平与游离HBsAg水平平行逐渐下降，并在其余23%的患者中持续存在。在 19% HBsAg-CIC 阳性患者中，复合 HBsAg 水平高于游离 HBsAg。 PEG-胰蛋白酶化结果与抗补体测定结果之间未观察到相关性。抗原过量的人工 HBsAg-IC 的抗互补性较差。大多数 (88%) HBsAg-CIC 阳性血清中 C3 [补体成分 3] 浓度正常或升高。抗原过量的 HBsAg-CIC 可能不太可能激活补体。该方法可用于HBsAg-CIC的临床研究和常规检查。
Using the PEG[polyethylene glycol]-trypsinization assay, HBsAg-CIC [hepatitis B surface antigen-circulating immune complexes] were detected in 70% of patients with acute hepatitis B, in 5% of the asymptomatic HBsAg carriers and in each of the 2 cases of chronic hepatitis B virus infection. In serial examinations, the complexed HBsAg levels in 77% of patients with acute hepatitis B decreased progressively in parallel with the free HBsAg levels, and persisted in the remaining 23%. In 19% of patients positive for HBsAg-CICs, the complexed HBsAg levels were higher than those of free HBsAg. No correlation was observed between results of PEG-trypsinization and an anticomplementary assay. Artificial HBsAg-IC in antigen excess were poorly anticomplementary. C3 [complement component 3] concentrations were normal or elevated in the majority (88%) of sera positive for HBsAg-CIC. HBsAg-CIC in antigen excess probably are unlikely to be complement-activating. The method is useful for clinical investigation and for routine examination for HBsAg-CIC.