Native plasmids of Fusobacterium nucleatum:: Characterization and use in development of genetic systems
Native plasmids of Fusobacterium nucleatum:: Characterization and use in development of genetic systems
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DOI：
10.1128/jb.182.4.1176-1180.2000
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发表时间：
2000-02-01
影响因子：
3.2
通讯作者：
Podkaminer, K
中科院分区：
文献类型：
--
作者：
Haake, SK;Yoder, SC;Podkaminer, K
对具核梭杆菌的三种天然质粒进行了表征，包括一种质粒pFN 1的DNA序列分析。穿梭质粒pHS 17能转化大肠杆菌和F. nucleatum ATCC 10953用pFN 1构建。pHS 17在F. nucleatum转化体，和转化效率的差异表明限制修饰系统的存在下，在F。核质
Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.