Prevalence of Group I Salmonella Kentucky in domestic food animals from Pennsylvania and overlap with human clinical CRISPR sequence types.
Prevalence of Group I Salmonella Kentucky in domestic food animals from Pennsylvania and overlap with human clinical CRISPR sequence types.
复制标题
宾夕法尼亚州家养食用动物中肯塔基州 I 类沙门氏菌的患病率以及与人类临床 CRISPR 序列类型的重叠。
DOI：
10.1111/zph.12506
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发表时间：
2018
影响因子：
2.4
通讯作者：
Shariat,NikkiW
中科院分区：
文献类型：
--
作者：
Rauch,HallieE;Vosik,Dorothy;Kariyawasam,Subhashinie;M'ikanatha,Nkuchia;Shariat,NikkiW
尽管很少与人类疾病相关，但肠沙门氏菌肯塔基肠亚种是美国报告的最常见的非临床非人类血清型，主要存在于家禽和家禽产品中，并且与牛有关。该血清型是多系的，根据 CRISPR 分型分析，可分为两组：I 组和 II 组。在从宾夕法尼亚州人类临床样本中分离出的肯塔基沙门氏菌中，两个谱系的代表性相同。这项研究的目的是确定这两个群体是否也存在于宾夕法尼亚州的家养食用动物中。我们分析了来自 67 个肯塔基州沙门氏菌分离株的 CRISPR 阵列，使用 PCR 和 CRISPR 阵列测序或全基因组序列分析来分析 CRISPR 阵列，并在 67 个肯塔基州沙门氏菌分离株（包括从农场、兽医临床样本以及零售肉类中收集的分离株）的集合中，我们发现肯塔基州沙门氏菌群是存在的独特谱系。我们发现，超过四分之一的动物分离株的特定亚型也被发现在同一地区、同一时期引起人类沙门氏菌病。
Although infrequently associated with illness in humans,Salmonella enterica, subsp.entericaserovar Kentucky is the most common non‐clinical, non‐human serovar reported in the United States, being largely found in poultry and poultry products, as well as being associated with cattle. This serovar is polyphyletic and can be separated into two groups, Group I and II, based on CRISPR‐typing analysis. InSalmonellaKentucky isolates from human clinical samples in Pennsylvania, both lineages are equally represented. The goal of this study was to determine whether both groups were also represented in domestic food animals in Pennsylvania. We analysed the CRISPR arrays from 67SalmonellaKentucky isolates used PCR and sequencing of CRISPR arrays or analysis of whole genome sequences to analyse the CRISPR arrays and Across a collection of 67SalmonellaKentucky isolates that includes those collected from farms, veterinary clinical samples as well as isolates from retail meats, we show that Group ISalmonellaKentucky are the exclusive lineage present. We reveal that the specific subtype of over a quarter of these animal isolates are also found to be responsible for causing human salmonellosis in the same region over the same time period.