[Protection and mechanisms of salvianolic-acid B on experimental renal interstitial fibrosis in rats].
[Protection and mechanisms of salvianolic-acid B on experimental renal interstitial fibrosis in rats].
复制标题
DOI：
--
复制
发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Hai-ying Lu;Juan Zhou;Min Lu;Yu-min Liu;Fei Wang;Min Lin;Yue Zhang
中科院分区：
文献类型：
--
作者：
Hai-ying Lu;Juan Zhou;Min Lu;Yu-min Liu;Fei Wang;Min Lin;Yue Zhang
目的探讨丹酚酸B （SA-B）对单侧输尿管梗阻（UUO）大鼠肾间质纤维化的影响及机制。方法18只雄性SD大鼠随机分为3组，每组6只。造模后给予SA-B治疗2周。采用光镜和电镜观察大鼠肾脏病理变化，采用Western blot和生物化学方法分析大鼠梗阻肾组织中α -平滑肌肌动蛋白（α - sma）和e -钙粘蛋白（E-cad）的表达水平。结果模型组肾脏病理检查显示肾小管管腔增宽，大量炎症细胞浸润，部分肾小管扩张，部分肾小管萎缩。小管上皮细胞核裂或核溶解，部分小管萎缩。α - sma蛋白表达量显著上调（p0.05）。结论SA-B对肾间质纤维化的拮抗作用主要是通过维持上皮表型，抑制进展性肾纤维化的主要作用细胞α - sma蛋白来实现的。
OBJECTIVE To investigate the effect and mechanism of salvianolic acid B (SA-B) on renal interstitial fibrosis in rats induced by unilateral ureteral obstruction (UUO). METHODS 18 male SD rats were randomly divided into 3 groups, 6 in each group. After the models were established, the rats were treated with SA-B for 2 weeks. Then their renal pathology were examined by hight microscope and electron microscopy Protein expression levels of alpha-smooth muscle actin (alpha-SMA) and E-cadherin (E-cad) in the obstructed kidney were analyzed by Western blot and Biochemistry assay. RESULTS Pathological examination of the kidney in model group showed tubules lumen widened and many inflammatory cells infiltrated, a part of renal tubule expanded and part of them atrophied. The tubular epithelial cells were karyorrhexis or karyolysis, some tubulars were atrophy. The protein expression of alpha-SMA were significantly up-regulated (P 0.05). CONCLUSION SA-B could antagonize renal interstitial fibrosis mainly by maintaining epithelial phenotype, inhibiting the protein of alpha-SMA which is the principal effect cells that are responsible for the progressive kidney fibrosis.