Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells
Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells
复制标题
L 和 HeLa 细胞对鹦鹉热衣原体和沙眼衣原体的寄生虫特异性吞噬作用
作者：
Gerald I. BYRNEt;J. Moulder
L细胞和HeLa 229细胞对鹦鹉热衣原体6BC株和沙眼衣原体性病淋巴肉芽肿440L株的吞噬作用发生的速率和程度比对大肠杆菌和聚苯乙烯乳胶球的吞噬作用大10至100倍。这两种衣原体都被它们以前从未遇到过的宿主细胞有效地吸收。衣原体的吞噬作用是由寄生虫表面配体与宿主细胞表面元素的相互作用引起的。衣原体配体容易被热变性，被抗体掩蔽，并且对蛋白酶和洗涤剂有抗性。宿主细胞组分被蛋白酶可逆地去除。当宿主细胞与放线菌酮孵育数小时时，衣原体的吞噬作用被抑制。有人认为，衣原体细胞表面上存在的配体与正常的，普遍存在的结构上的宿主细胞的表面上的高亲和力是一种进化适应细胞内的存在。术语寄生虫特异性吞噬作用用于描述非专职吞噬细胞对衣原体的有效吞噬作用，并将其与宿主特异性免疫和非免疫吞噬作用区分开来。
Phagocytosis of the 6BC strain of Chlamydia psittaci and the lymphogranuloma venereum 440L strain of Chlamydia trachomatis by L cells and HeLa 229 cells occurred at rates and to extents that were 10 to 100 times greater than those observed for the phagocytosis of Escherichia coli and polystyrene latex spheres. Both species of Chlamydia were efficiently taken up by host cells of a type they had not previously encountered. Phagocytosis of chlamydiae was brought about by the interaction of parasite surface ligands with elements of the host cell surface. The chlamydial ligands were readily denatured by heat, were masked by antibody, and were resistant to proteases and detergents. The host cell components were reversibly removed by proteases. Chlamydial phagocytosis was inhibited when host cells were incubated for many hours with cycloheximide. It was suggested that the presence on the chlamydial cell surface of ligands with high affinity for normal, ubiquitously occurring structures on the surface of host cells is an evolutionary adaptation to intracellular existence. The term parasite-specified phagocytosis was used to describe the efficient phagocytosis of chlamydiae by nonprofessional phagocytes and to distinguish it from the host-specified immunological and non-immunological phagocytosis carried out by professional phagocytes.