Evaluation of the CellaVision DM96 advanced RBC application for screening and follow-up of malaria infection
Evaluation of the CellaVision DM96 advanced RBC application for screening and follow-up of malaria infection
复制标题
DOI：
10.1016/j.diagmicrobio.2017.12.002
复制
发表时间：
2018-04-01
影响因子：
2.9
通讯作者：
Emmerechts, Jan
中科院分区：
文献类型：
--
作者：
Florin, Lisa;Maelegheer, Karel;Emmerechts, Jan
CellaVision DM 96是一种数字细胞形态学系统，用于白色和红细胞的自动分类。CellaVision Advanced RBC应用程序（ARBCA）将RBC预分类为21个类别，包括寄生RBC，并允许操作员重新分类。在这项研究中，疟疾的检测和计算寄生虫血症的软件的性能进行了评估，并与显微镜（n = 40）。对于CellaVision，评价了重新分类前后的结果。由于分析的RBC数量较少和照片分辨率有限，即使在重新分类后（72%），灵敏度也是中等的。根据Passing-Bablok分析，CellaVision结果与显微镜检查结果相关，CellaVision的值略低。运行内、运行间和观察员间变异性可接受。CellaVision ARBCA的低灵敏度使其无法用作疟疾筛查技术。然而，由于其良好的相关性与显微镜和短周转时间，它可能是有用的后续寄生虫血症。需要更大规模的研究来证实这些发现。(C)2017爱思唯尔公司All rights reserved.
CellaVision DM96 is a digital cell morphology system for automated classification of white and red blood cells. CellaVision Advanced RBC application (ARBCA) pre-classifies RBC in 21 categories, including parasitized RBC, and allows re-classification by the operator. In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n = 40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs. CellaVision results correlated with microscopy according to Passing-Bablok analysis, with slightly lower values for CellaVision. Within-run, between-run and inter-observer variability were acceptable. The low sensitivity of CellaVision ARBCA precludes its use as a screening technique for malaria. However, due to its good correlation with microscopy and short turnaround-times, it may be useful in follow-up of parasitemia. Larger studies are required to confirm these findings. (C) 2017 Elsevier Inc. All rights reserved.