Phylogenetic relationships in the grass family (Poaceae) based on the nuclear single copy locus topoisomerase 6 compared with chloroplast DNA
Phylogenetic relationships in the grass family (Poaceae) based on the nuclear single copy locus topoisomerase 6 compared with chloroplast DNA
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DOI：
10.1080/14772000.2014.890137
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发表时间：
2014-01-02
影响因子：
1.9
通讯作者：
Roeser, Martin
中科院分区：
文献类型：
--
作者：
Blaner, Anne;Schneider, Julia;Roeser, Martin
利用新获得的核单拷贝基因拓扑异构酶6 （Topo6）横跨8-11四个外显子的位点和叶绿体matK基因，研究了草科植物的系统发育关系。使用最大简约、最大似然和贝叶斯方法对数据进行评估。所有的分析都表明，链chaeta属和Anomochloa属是早期分化的，其次是Pharus属，是Poaceae的姐妹，并且核数据支持了anomochloideae亚科的单系性。在Topo6分析中，剩余的禾本科形成了一个强支持的单系类群，分为主要分支BEP和PACMAD。Topo6数据有力地支持了BEP进化支的单一性。结果表明，两组数据存在明显的不一致性，如竹科、竹科和竹科的亚科关系存在差异。大多数被分析的物种是Pooideae亚科的代表，通过另一个Topo6区域跨越外显子17-19的PCR片段长度差异对其进行了更详细的分析。matK数据有力地支持了Pooideae的单系性，而核数据将Brachyelytrum置于剩余的Pooideae之外。在系统发育树中，早期进化谱系的关系在很大程度上仍未得到解决，但“核心”Pooideae （Aveneae/Poeae部落综合体和Hordeeae）在所有分析中都得到了高度支持。扩增长度的差异说明了Pooideae的部落和亚部落分类。新研究的Topo6区域相对保守的结构使其成为核基因组中一个有希望的标记，可以成功地进行pcr扩增，以研究禾草中更高水平的系统发育关系，也许还可以研究禾草目中不同科之间的系统发育关系。
Phylogenetic relationships within the grass family were studied using a newly obtained locus of the nuclear single copy gene topoisomerase 6 (Topo6) spanning the four exons 8-11 and the chloroplast matK gene. Data were evaluated using maximum parsimony, maximum likelihood and Bayesian methods. All analyses showed genera Streptochaeta and Anomochloa as early diverging, followed by Pharus as sister to the rest of the Poaceae, and monophyly of the subfamily Anomochlooideae was supported by the nuclear dataset. The remaining grasses formed a strongly supported and monophyletic group, which split into the major clades BEP and PACMAD in the Topo6 analyses. Monophyly of the BEP clade was strongly supported by the Topo6 data. The results showed clearly incongruity between the two sets of data, such as the different subfamilial relationships of Bambusoideae, Ehrhartoideae and Pooideae. Most of the analysed species are representatives of subfamily Pooideae, which was analysed in more detail by PCR fragment length differences of another Topo6 region spanning the exons 17-19. Monophyly of Pooideae was strongly supported by the matK data, whereas the nuclear data placed Brachyelytrum outside of the remaining Pooideae. Relationships within the early evolutionary lineages remained largely unresolved in the phylogenetic trees, but the 'core' Pooideae (Aveneae/Poeae tribe complex and Hordeeae) were highly supported in all analyses. The differences in amplification lengths illustrate the tribe and subtribe classification of Pooideae. The comparatively conserved structure of the newly studied Topo6 region makes it a promising marker from the nuclear genome that could be successfully PCR-amplified to study higher-level phylogenetic relationships within grasses and perhaps between families within the order Poales.