OsWRKY53 Promotes Abscisic Acid Accumulation to Accelerate Leaf Senescence and Inhibit Seed Germination by Downregulating Abscisic Acid Catabolic Genes in Rice.
OsWRKY53 Promotes Abscisic Acid Accumulation to Accelerate Leaf Senescence and Inhibit Seed Germination by Downregulating Abscisic Acid Catabolic Genes in Rice.
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DOI：
10.3389/fpls.2021.816156
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发表时间：
2021
影响因子：
5.6
通讯作者：
Yuan M
中科院分区：
文献类型：
--
作者：
Xie W;Li X;Wang S;Yuan M
脱落酸（阿坝）在很大程度上促进植物叶片衰老和抑制种子萌发。内源阿坝含量是由许多转录因子微调。在这项研究中，我们表明OsWRKY 53是水稻叶片衰老的正调节因子和种子萌发的负调节因子。OsWRKY 53在老化、黑暗和阿坝处理下的叶片中被诱导表达。OsWRKY 53过表达（OsWRKY 53-oe）的植株在自然衰老、黑暗诱导衰老和ABA诱导衰老条件下表现出较早的黄化叶片，而OsWRKY 53（oswrky 53）敲除突变体在自然衰老、黑暗诱导衰老和ABA诱导衰老条件下比野生型保持绿色叶片。转录分析表明，OsWRKY 53-oe叶片中阿坝分解代谢基因OsABA 8 ox 1和OsABA 8 ox 2表达显著下调。染色质免疫沉淀和原生质体瞬时表达分析表明，OsWRKY 53直接与OsABA 8 ox 1和OsABA 8 ox 2的启动子结合，抑制其转录，导致内源阿坝含量升高，从而促进OsWRKY 53-oe植株叶片的早衰。说明OsWRKY 53是通过调节阿坝积累促进叶片衰老的正调控因子。此外，积累的阿坝同时抑制OsWRKY 53-oe植物的种子萌发和萌发后生长。综上所述，OsWRKY 53通过抑制阿坝分解代谢基因的转录，促进阿坝的积累，从而调节ABA诱导的叶片衰老和ABA介导的种子萌发抑制。
Abscisic acid (ABA) largely promotes leaf senescence and inhibits seed germination in plants. Endogenous ABA content is finely tuned by many transcription factors. In this study, we showed that OsWRKY53 is a positive regulator of leaf senescence and a negative regulator of seed germination in rice. OsWRKY53 expression was induced in leaves under aging, dark, and ABA treatment. The OsWRKY53-overexpressing (OsWRKY53-oe) plants showed early yellowing leaves, while the OsWRKY53 (oswrky53) knockout mutants maintained green leaves than the wild type under natural, dark-induced, and ABA-induced senescence conditions. Transcriptional analysis revealed that ABA catabolic genes, namely, OsABA8ox1 and OsABA8ox2, two key genes participating in ABA catabolism harboring ABA 8′-hydroxylase activity, were markedly downregulated in OsWRKY53-oe leaves. Chromatin immunoprecipitation and protoplast transient assays revealed that OsWRKY53 directly bound to the promoters of OsABA8ox1 and OsABA8ox2 to repress their transcription, resulting in elevated endogenous ABA contents that promoted premature leaf senescence in the OsWRKY53-oe plants. It indicates that OsWRKY53 is a positive regulator through regulating ABA accumulation to promote leaf senescence. In addition, accumulated ABA simultaneously inhibited seed germination and post-germination growth in OsWRKY53-oe plants. Taken together, OsWRKY53 suppresses the transcript of ABA catabolic genes to promote ABA accumulation to modulate ABA-induced leaf senescence and ABA-mediated inhibition of seed germination.
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影响因子：
7.2
作者：
Morita, Ryouhei;Sato, Yutaka;Kusaba, Makoto
通讯作者：
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作者：
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