Tussah Silk Fibroin Excels Silk Fibroin from the Domesticated Silkworm in Supporting the Development of Neurons
Tussah Silk Fibroin Excels Silk Fibroin from the Domesticated Silkworm in Supporting the Development of Neurons
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DOI：
10.1007/978-3-642-14515-5_401
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发表时间：
2010
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影响因子：
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通讯作者：
Jing Qu;L. Xin;Xiaojing Xu;F. Zhang;B. Zuo;Huanxiang Zhang
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文献类型：
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作者：
Jing Qu;L. Xin;Xiaojing Xu;F. Zhang;B. Zuo;Huanxiang Zhang
以生物材料为材料的人工神经假体在神经组织工程中具有广阔的应用前景。丝素蛋白是一种来源于家蚕的纤维状蛋白质，由于其与多种细胞的生物相容性而被广泛研究。然而，与桑蚕丝素蛋白（SF）相比，柞蚕丝素蛋白（TSF）具有Ala、Asp和Arg含量高，Gly含量低，Arg-Gly-Asp（RGD）三肽序列存在等特点，其与神经细胞之间的相互作用尚不清楚。在这项研究中，我们分析了神经元的生长和发育的薄膜或静电纺非织造纳米纤维的桑蚕丝素蛋白（SF）和TSF。神经元来源于从新生大鼠脑室下区（SVZ）纯化的细胞的低密度原代培养物。测定了丝素蛋白材料上神经元的一级树突数、分支数和树突长度。结果表明，TSF丝素蛋白支架是最有利于神经元附着和生长的丝素蛋白材料，TSF比SF更有利于神经元的发育，提示TSF在制备治疗神经损伤或疾病的组织工程神经导管方面具有潜在的应用价值。
Artificial prostheses that are made of biomaterials hold great promise in nerve tissue engineering for the regeneration of nervous system after injury. Silk fibroin, a fibrous protein from the domesticated silkworm, Bombyx mori, has been studied extensively for its biocompatibility with a variety of cells. However, little is known about the tussah silk fibroin (TSF), a type of wild silks that, compared with Bombyx mori silk fibroin (SF), is characterized by more Ala, Asp and Arg contents, and less Gly, and the presence of tripeptide sequence Arg-Gly-Asp (RGD), and the interactions between SF, TSF and nerve cells. In this study we analyzed the growth and development of neurons on films or electrospun non-woven nanofibers of Bombyx mori silk fibroin (SF) and TSF. Neurons were derived in a low-density primary cultures of cells purified from the subventricular zone (SVZ) of newborn rat brain. The numbers of primary dendrites and branches as well as dendritic length of neurons on silk fibroin materials were measured. Results showed that TSF silk fibroin nanofiber is the most favorable silk fibroin material for supporting the attachment and growth of neurons and that TSF is more compatible for the development of neurons than SF, suggesting a potential use of TSF for preparing the tissue-engineered nerve guides to treat nerve injuries or diseases.