Phylogenomic analyses reveal subclass Scuticociliatia as the sister group of subclass Hymenostomatia within class Oligohymenophorea
Phylogenomic analyses reveal subclass Scuticociliatia as the sister group of subclass Hymenostomatia within class Oligohymenophorea
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系统发育分析表明，Scuticociliatia 亚纲是 Oligohymenophorea 纲 Hymenostomatia 亚纲的姐妹群
DOI：
10.1016/j.ympev.2015.05.007
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发表时间：
2015-09-01
影响因子：
4.1
通讯作者：
Miao, Wei
中科院分区：
文献类型：
--
作者：
Feng, Jin-Mei;Jiang, Chuan-Qi;Miao, Wei
Scuticociliates和hymenostomes是纤毛虫纲Oligohydrhorea的两个类群，Oligohydrhorea是一个多样化的分支，包括两个模式属，四膜虫属和草履虫属，由于它们易于培养和适应广泛的生物化学和遗传学调查，已被深入研究。然而，Oligohydrophorea的亚类之间的系统发育关系，特别是Scuticociliatia和Hymenostomatia之间，没有明确解决。在这里，我们调查Scuticociliatia和Hymenostomatia亚类之间的系统发育关系的基础上组学数据。五个物种的转录组，包括四个oligohyrhoreans和一个colpodean，进行了测序。基于来自26个分类群的113个编码43，528个氨基酸残基的基因构建了一个超矩阵，其中包括10个代表性的Oligohydrophorea。我们的基因组分析显示，单系Scuticociliatia是姐妹单系Hymenostomatia，它们一起形成的终端分支内的单系类Oligohyphyphorea。拓扑测试拒绝了这种关系的竞争假设。我们的研究结果提供了确凿的证据Scuticociliatia和Hymenostomatia之间的密切关系，证明可能使用的模式hymenostomeT。本研究为盾纤毛虫分子和细胞生物学研究提供了一个有效的实验系统。(C)2015 Elsevier Inc. All rights reserved.
Scuticociliates and hymenostomes are two groups of the ciliate class Oligohymenophorea, a diverse clade that includes two model genera, Tetrahymena and Paramecium, which have been intensively studied due to their ease of culture and their amenability to a wide range of biochemical and genetic investigations. However, phylogenetic relationships among the subclasses of the Oligohymenophorea, and especially between the Scuticociliatia and Hymenostomatia, are not clearly resolved. Here, we investigate the phylogenetic relationship between the subclasses Scuticociliatia and Hymenostomatia based on omics data. The transcriptomes of five species, comprising four oligohymenophoreans and one colpodean, were sequenced. A supermatrix was constructed for phylogenomic analyses based on 113 genes encoding 43,528 amino acid residues from 26 taxa, including ten representatives of the class Oligohymenophorea. Our phylogenomic analyses revealed that the monophyletic Scuticociliatia is sister to the monophyletic Hymenostomatia, which together form the terminal branch within the monophyletic class Oligohymenophorea. Competing hypotheses for this relationship were rejected by topological tests. Our results provide corroborative evidence for the close relationship between the subclasses Scuticociliatia and Hymenostomatia, justifying the possible use of the model hymenostome T. thermophila as an effective experimental system to study the molecular and cellular biology of the scuticociliates. (C) 2015 Elsevier Inc. All rights reserved.