NEUROGENESIS IS ABSENT IN THE BRAINS OF ADULT HONEY-BEES AND DOES NOT EXPLAIN BEHAVIORAL NEUROPLASTICITY
NEUROGENESIS IS ABSENT IN THE BRAINS OF ADULT HONEY-BEES AND DOES NOT EXPLAIN BEHAVIORAL NEUROPLASTICITY
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DOI：
10.1016/0304-3940(95)11913-h
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发表时间：
1995-09-08
影响因子：
2.5
通讯作者：
ROBINSON, GE
中科院分区：
文献类型：
--
作者：
FAHRBACH, SE;STRANDE, JL;ROBINSON, GE
蘑菇体是最常与学习有关的昆虫大脑结构，在蜜蜂成虫行为发育过程中表现出结构可塑性。我们通过用5-溴-2‘-脱氧尿苷(BrdU)标记复制细胞的DNA来研究成年神经发生是否有助于蘑菇体的可塑性。对喂食或注射BrdU的蜜蜂以及体外用BrdU处理的蜜蜂的脑切片进行的免疫细胞化学分析显示，无论工蜂的年龄或行为状态(1日龄、护士或觅食者)，都没有标记的神经元核。我们的结果表明，成年蜜蜂大脑中的神经发生是一种罕见的事件，如果它真的发生的话。因此，在成年行为发育过程中观察到的蜜蜂大脑的结构变化必须用发育过程来解释，而不是神经发生。
The mushroom bodies, the insect brain structures most often associated with learning, exhibit structural plasticity during adult behavioral development in honey bees. We have investigated whether adult neurogenesis contributes to the plasticity of the mushroom bodies by labeling the DNA of replicating cells with 5-bromo-2'-deoxyuridine (BrdU). Immunocytochemical analysis of brain sections from bees fed or injected with BrdU as well as from bees treated in vitro with BrdU revealed no labeled neuronal nuclei, regardless of age or behavioral status of the worker bee (1-day old, nurse, or forager). Our results demonstrate that neurogenesis in the adult bee brain is a rare event, if it occurs at all. Therefore, the structural changes observed in the bee brain during adult behavioral development must be explained by developmental processes other than neurogenesis.