Vascular Architecture Generates Fine Scale Variation in Systemic Induction of Proteinase Inhibitors in Tomato
Vascular Architecture Generates Fine Scale Variation in Systemic Induction of Proteinase Inhibitors in Tomato
复制标题
番茄中蛋白酶抑制剂的系统诱导中血管结构产生精细尺度变化
DOI：
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发表时间：
2000
影响因子：
2.3
通讯作者：
R. Ricco
中科院分区：
文献类型：
--
作者：
C. Orians;Jason M Pomerleau;R. Ricco
在许多植物物种中发现了损伤后的系统诱导。尽管这种普遍赞赏的重要性，感应，很少有研究的特点空间变异的感应。我们用番茄，番茄，研究如何损害一个单一的叶子影响的空间分布的系统诱导的蛋白酶抑制在受损的叶子。我们粉碎每个小叶的第二真叶用镊子和测量的空间分布的蛋白酶抑制剂在叶3，4，和5在8，16，24，48，72，和120小时。蛋白酶抑制剂活性的组成水平进行了定量在未受损的植物。我们假设，由于信号运动的血管控制，系统诱导将显示叶间和叶内的变异性。叶2受损后，诱导在叶5中最明显，在叶3中最小。一般来说，蛋白酶抑制剂活性在24小时最大，然后下降。与维管结构预测的结果一致，相邻的正序排列中近叶侧的诱导率高于远叶侧。在未受损的邻近植物中蛋白酶抑制剂活性没有增加。总的来说，我们的研究结果表明，系统诱导蛋白酶抑制剂部分控制的维管结构和系统诱导的未来研究应检查正在研究的植物的维管结构。我们认为，这种空间变化可能会影响性能的草食动物敏感的诱导化学变化。
Systemic induction following damage has been found in many plant species. Despite this widespread appreciation for the importance of induction, few studies have characterized the spatial variability of induction. We used tomato, Lycopersicon esculentum, to examine how damage to a single leaf affected the spatial distribution of systemic induction of proteinase inhibition in leaves above the damaged leaf. We crushed each leaflet of the second true leaf with forceps and measured the spatial distribution of proteinase inhibition in leaves 3, 4, and 5 at 8, 16, 24, 48, 72, and 120 hr. Constitutive levels of proteinase inhibitor activity were quantified in undamaged plants. We hypothesized that, due to vascular control of signal movement, systemic induction would show both among and within leaf variability. Following damage to leaf 2, induction was most pronounced in leaf 5 and minimal in leaf 3. In general, proteinase inhibitor activity was greatest at 24 hr and then declined. As predicted by vascular architecture, the near side of leaves in adjacent orthostichies showed higher induction than the far side of leaves. There was no increase in proteinase inhibitor activity in the undamaged neighboring plants. Overall our results demonstrate that systemic induction of proteinase inhibitors is partially controlled by vascular architecture and that future studies on systemic induction should examine the vascular architecture of the plants being studied. We argue that this spatial variation may influence the performance of herbivores sensitive to induced chemical changes.
DOI：
10.1073/pnas.89.6.2394
发表时间：
1992-03-15
影响因子：
11.1
作者：
PROCHASKA, HJ;SANTAMARIA, AB;TALALAY, P
通讯作者：
TALALAY, P