TROPHIC EFFECT OF BETA-AMYLOID PRECURSOR PROTEIN ON CEREBRAL CORTICAL-NEURONS IN CULTURE
TROPHIC EFFECT OF BETA-AMYLOID PRECURSOR PROTEIN ON CEREBRAL CORTICAL-NEURONS IN CULTURE
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DOI：
10.1016/s0006-291x(05)81412-3
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发表时间：
1991-11-27
影响因子：
3.1
通讯作者：
KIMURA, J
中科院分区：
文献类型：
--
作者：
ARAKI, W;KITAGUCHI, N;KIMURA, J
我们研究了人β-淀粉样前体蛋白(APP)对无血清培养的原代大鼠大脑皮质神经元的影响。将编码APP770和APP695 N端片段的APP cDNA导入COS-1细胞，可产生两种分泌APP770和APP695的APP667和APP592。这两种高度纯化的APP在加入到培养液中时，都以剂量依赖的方式促进神经元存活和轴突延伸，在大约100 NM时效果最好。这些结果表明，分泌形式的APP对大脑皮层神经元具有营养活性。
We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons.