Enhanced expression of human immunodeficiency virus type 1 correlates with development of AIDS.
Enhanced expression of human immunodeficiency virus type 1 correlates with development of AIDS.
复制标题
人类免疫缺陷病毒 1 型的增强表达与艾滋病的发展相关。
DOI：
10.1006/viro.1993.1514
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Rinaldo,C
中科院分区：
文献类型：
--
作者：
Gupta,P;Kingsley,L;Armstrong,J;Ding,M;Cottrill,M;Rinaldo,C
艾滋病的进展可能与人类免疫缺陷病毒1型（HIV-1）的复制水平显著相关。我们使用定量细胞培养和定量DNA和RNA PCR来测量外周血单个核细胞中的病毒载量和表达，这些细胞是从参加多中心艾滋病队列研究的hiv -1血清阳性同性恋男性中横断面和纵向获得的。我们的研究结果表明，产生HIV-1的循环CD4+ t淋巴细胞的数量随着CD4+ t细胞总数的减少而增加。然而，产生hiv -1的CD4+细胞的数量与感染的持续时间之间没有相关性。此外，HIV-1 gag RNA水平随着疾病进展和CD4+细胞数量下降而升高。然而，无症状且CD4+细胞计数稳定的受试者在整个随访期间（38-71个月）保持非常低水平的HIV-1 RNA表达。与病毒RNA表达相反，随着疾病的进展，前病毒DNA的水平没有显著变化。然而，艾滋病患者的前病毒DNA水平明显高于无症状男性。这种增加的HIV-1 DNA水平是在艾滋病发展前34-68个月检测到的。这些结果支持HIV-1 RNA表达在艾滋病发展中的作用。
The progression to AIDS may be significantly related to the level of human immunodeficiency virus type 1 (HIV-1) replication. We have used quantitative cell culture and quantitative DNA and RNA PCR to measure viral load and expression in peripheral blood mononuclear cells obtained cross-sectionally and longitudinally from HIV-1-seropositive homosexual men enrolled in the Multicenter AIDS Cohort Study. Our results indicate that the number of circulating CD4+T-lyrnphocytes producing HIV-1 increased as the total number of CD4+T-cells declined. However, there was no correlation between the number of HIV-1-producing CD4+cells and the duration of infection. Furthermore, the level of HIV-1 gag RNA increased as the disease progressed and CD4+cell numbers declined. Subjects who remained asymptomatic with stable CD4+cell counts, however, maintained a very low level of HIV-1 RNA expression during the entire period of follow-up (38-71 months). In contrast to viral RNA expression, the level of proviral DNA did not change significantly as the disease progressed. However, the level of proviral DNA was significantly higher in AIDS patients than in men who remained asymptomatic. Such increased levels of HIV-1 DNA were detected 34-68 months before the development of AIDS. These results support the role of HIV-1 RNA expression in the development of AIDS.