Human CD16+ and CD16- monocyte subsets display unique effector properties in inflammatory conditions in vivo
Human CD16+ and CD16- monocyte subsets display unique effector properties in inflammatory conditions in vivo
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DOI：
10.1189/jlb.0111022
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发表时间：
2011-12-01
影响因子：
5.5
通讯作者：
Sanchez-Torres, Carmen
中科院分区：
文献类型：
--
作者：
Aguilar-Ruiz, Sergio R.;Torres-Aguilar, Honorio;Sanchez-Torres, Carmen
基于CD 14和CD 16表达鉴定了人Mo的两个主要亚群：经典的CD 16（-）Mo和次要的CD 14（+）CD 16（+）Mo。体外研究表明，每个细胞群具有不同的功能和分化潜力。然而，这些发现的体内相关性仍不清楚。为了评估人Mo在体内模型中的发育和功能，我们将两种Mo亚群转移到处于稳态或炎症条件下的免疫受损小鼠的腹膜中。用可溶性LPS或颗粒状酵母聚糖诱导炎症。在酵母多糖转染后早期，CD 16（+）比CD 16（-）Mo具有更强的吞噬作用，产生更高量的TNF和IL-6。他们还在任何评估条件下产生更高水平的β 2-防御素，这可能代表了该亚群的新标志物。与此相反，分化CD 16（-）Mo（转移后24小时）获得更大的APC能力在LPS诱导的腹膜炎，而没有一个Mo亚群获得这种能力与酵母多糖。CX（3）CL 1支持两种Mo亚群在体内的存活。在人类腹膜炎中存在类似的Mo亚群。这些结果支持Mo亚群的专门作用的想法，其中CD 16（+）可能在立即的先天免疫应答中起作用，而CD 16（-）可能作为APC起主要作用。J. Leukoc. 90：1119-1131; 2011.
Two major subsets of human Mo are identified based on CD14 and CD16 expression: the classical CD16(-) Mo and the minor CD14(+)CD16(+) Mo. In vitro studies suggested distinct function and differentiation potential for each cell population. However, the in vivo relevance of these findings remains unclear. To evaluate the development and function of human Mo in an in vivo model, we transferred both Mo subpopulations into the peritoneum of immunocompromised mice in homeostatic or inflammatory conditions. Inflammation was induced with soluble LPS or particulate zymosan. CD16(+) were more phagocytic and produced higher amounts of TNF and IL-6 than CD16(-) Mo early after transfer with zymosan. They also produced higher levels of beta 2-defensin in any condition evaluated, which could represent a new marker for this subpopulation. In contrast, differentiating CD16(-) Mo (24 h after transfer) acquired greater APC capacity in LPS-induced peritonitis, whereas none of the Mo subsets attained this ability with zymosan. CX(3)CL1 supported the survival of both Mo subsets in vivo. Similar Mo subpopulations were present in human peritonitis. These results support the idea of specialized roles of the Mo subset, where CD16(+) might act in an immediate innate immune response, whereas CD16(-) could have a major role as APCs. J. Leukoc. Biol. 90: 1119-1131; 2011.