Development and Application of a Near-Infrared Fluorescence Probe for Oxidative Stress Based on Differential Reactivity of Linked Cyanine Dyes
Development and Application of a Near-Infrared Fluorescence Probe for Oxidative Stress Based on Differential Reactivity of Linked Cyanine Dyes
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DOI：
10.1021/ja910090v
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发表时间：
2010-03-03
影响因子：
15
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
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作者：
Oushiki, Daihi;Kojima, Hirotatsu;Nagano, Tetsuo
活性氧（Reactive oxygen species，ROS）作为信号分子在多种生理条件下发挥作用，ROS的过度产生参与了多种疾病的发病机制。因此，用于可视化ROS的荧光探针是有前途的工具，用它来揭示生理和病理过程的分子机制，也可能是有用的诊断。在这里，我们描述了一种新的荧光探针，FOSCY-1，在生理上有利的近红外区工作。该探针由两个不同的ROS反应性花青染料连接的接头;反应的更敏感的染料与ROS释放分子内荧光猝灭的不太敏感的染料。我们成功地应用该探针检测由HL 60细胞和猪中性粒细胞产生的ROS，并在腹膜炎小鼠模型中成像氧化应激。
Reactive oxygen species (ROS) operate as signaling molecules under various physiological conditions, and overproduction of ROS is involved in the pathogenesis of many diseases. Therefore, fluorescent probes for visualizing ROS are promising tools with which to uncover the molecular mechanisms of physiological and pathological processes and might also be useful for diagnosis. Here we describe a novel fluorescence probe, FOSCY-1, operating in the physiologically favorable near-infrared region. The probe consists of two differentially ROS-reactive cyanine dyes connected by a linker; reaction of the more susceptible dye with ROS releases intramolecular fluorescence quenching of the less susceptible dye. We successfully applied this probe to detect ROS produced by HL60 cells and porcine neutrophils and for imaging oxidative stress in a mouse model of peritonitis.