Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C-elegans
Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C-elegans
复制标题
DOI：
10.1016/j.cell.2006.10.040
复制
发表时间：
2006-12-15
期刊：
影响因子：
64.5
通讯作者：
Bartel, David P.
中科院分区：
文献类型：
--
作者：
Ruby, J. Graham;Jan, Calvin;Bartel, David P.
我们从秀丽隐杆线虫中测序了40万个小RNA。另外18个microRNA（miRNA）基因被鉴定，从而将我们在C.优雅的。还观察到由RNA指导的RNA聚合酶产生的数千种内源性siRNA，其优先作用于与精子发生和转座子相关的转录物。此外，还发现了第三类线虫小RNA，称为21 U-RNA。21个U-RNA精确地为21个核苷酸长，开始于尿苷5 '-单磷酸，但在其剩余的20个核苷酸中是不同的，并且在其3'-末端核糖处出现修饰。21个U-RNA来源于5700多个基因组位点，分布在IV号染色体的两个广泛区域，主要位于蛋白质编码基因之间或其内含子内。这些基因座共享一个大的上游基序，使得能够准确预测额外的21个U-RNA。该基序在其他线虫中是保守的，大概是因为它对产生这些多样的、自主表达的小RNA（dasRNA）的重要性。
We sequenced 400,000 small RNAs from Caenorhabditis elegans. Another 18 microRNA (miRNA) genes were identified, thereby extending to 112 our tally of confidently identified miRNA genes in C. elegans. Also observed were thousands of endogenous siRNAs generated by RNA-directed RNA polymerases acting preferentially on transcripts associated with spermatogenesis and transposons. In addition, a third class of nematode small RNAs, called 21 U-RNAs, was discovered. 21 U-RNAs are precisely 21 nucleotides long, begin with a uridine 5'-monophosphate but are diverse in their remaining 20 nucleotides, and appear modified at their 3'-terminal ribose. 21 U-RNAs originate from more than 5700 genomic loci dispersed in two broad regions of chromosome IV-primarily between protein-coding genes or within their introns. These loci share a large upstream motif that enables accurate prediction of additional 21 U-RNAs. The motif is conserved in other nematodes, presumably because of its importance for producing these diverse, autonomously expressed, small RNAs (dasRNAs).