Detection of Δ9-tetrahydrocannabinolic acid A in human urine and blood serum by LC-MS/MS
Detection of Δ9-tetrahydrocannabinolic acid A in human urine and blood serum by LC-MS/MS
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DOI：
10.1002/jms.1167
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发表时间：
2007-03-01
影响因子：
2.3
通讯作者：
Weinmann, Wolfgang
中科院分区：
文献类型：
--
作者：
Jung, Julia;Kempf, Juergen;Weinmann, Wolfgang
9-四氢大麻酚酸A （Delta 9-THCA-A）是大麻植物中Delta 9-四氢大麻酚（Delta 9-THC）的前体。在吸烟过程中，非精神活性的Delta 9-THCA-A转化为Delta 9-THC，这是大麻和哈希什的主要精神活性成分。虽然Delta 9-THCA-A到Delta 9-THC的脱羧被认为是完全的——这意味着在大麻消费者的尿液和血清中不应该检测到Delta 9-THCA-A——但我们在警方控制的怀疑在药物影响下驾驶的司机（DUID）的尿液和血清样本中发现了Delta 9-THCA-A。采用固相萃取法制备尿液和血清样品，进行LC-MS/MS分析。用苯己基柱进行分析，乙腈梯度洗脱。为了检测Delta 9-THCA-A，质谱仪（SCIEX API 365三重四极杆质谱仪，TurbolonSpray源）在多反应监测（MRM）模式下运行，使用以下转换：m/z 357 - bbb313， m/z 357 - >45和m/z 357 - >91。在一些大麻消费者的尿液和血清样本中可以检测到δ 9-三氢乙酸- a，其浓度在尿液中高达10.8纳克/毫升，在血清中高达14.8纳克/毫升。大多数血清样品中δ 9-THCA-A的浓度低于δ 9-THC的浓度，导致δ 9-THCA-A/ δ 9-THC的摩尔比约为5.0-18.6%。只有在一种情况下，假设最后一次摄入和血液采样之间的时间很短，血清中的摩尔比为18.6%。这表明消除动力学的差异，这需要详细研究。版权所有(c) 2007约翰威利父子有限公司
9-Tetrahydrocannabinolic acid A (Delta 9-THCA-A) is the precursor of Delta 9-tetrzthydrocannabinol (Delta 9-THC) in hemp plants. During smoking, the non-psychoactive Delta 9-THCA-A is converted to Delta 9-THC, the main psychoactive component of marihuana and hashish. Although the decarboxylation of Delta 9-THCA-A to Delta 9-THC was assumed to be complete - which means that no Delta 9-THCA-A should be detectable in urine and blood serum of cannabis consumers - we found Delta 9-THCA-A in the urine and blood serum samples collected from police controls of drivers suspected for driving under the influence of drugs (DUID). For LC-MS/MS analysis, urine and blood serum samples were prepared by solid-phase extraction. Analysis was performed with a phenylhexyl column using gradient elution with acetonitrile. For detection of Delta 9-THCA-A, the mass spectrometer (MS) (SCIEX API 365 triple-quadrupole MS with TurbolonSpray source) was operated in the multiple reaction monitoring (MRM) mode using the following transitions: m/z 357 -> 313, m/z 357 -> 245 and m/z 357 -> 191. Delta 9-THCA-A could be detected in the urine and blood serum samples of several cannabis consumers in concentrations of up to 10.8 ng/ml in urine and 14.8 ng/ml in serum. The concentration of Delta 9-THCA-A was below the Delta 9-THC concentration in most serum samples, resulting in molar ratios of Delta 9-THCA-A/Delta 9-THC of approximately 5.0-18.6%. Only in one case, where a short elapsed time between the last intake and blood sampling is assumed, the molar ratio was 18.6% in the serum. This indicates differences in elimination kinetics, which need to be investigated in detail. Copyright (c) 2007 John Wiley & Sons, Ltd.