Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling.
Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling.
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DOI：
10.1016/j.bmc.2011.12.043
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发表时间：
2012-03
影响因子：
3.5
通讯作者：
Katsunori Tanaka;Satomi Yokoi;Koji Morimoto;Takayuki Iwata;Y. Nakamoto;K. Nakayama;K. Kôyama;Takeshi Fujiwara;K. Fukase
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文献类型：
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作者：
Katsunori Tanaka;Satomi Yokoi;Koji Morimoto;Takayuki Iwata;Y. Nakamoto;K. Nakayama;K. Kôyama;Takeshi Fujiwara;K. Fukase
建立了多用途的活细胞标记方法。细胞表面最初通过氮杂电环化生物素化，然后用荧光标记的亲和素或抗生物素抗体处理。
Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.