Semi-preparative HPLC purification of δ-tocotrienol (δ-T3) from Elaeis guineensis Jacq. and Bixa orellana L. and evaluation of its in vitro anticancer activity in human A375 melanoma cells
Semi-preparative HPLC purification of δ-tocotrienol (δ-T3) from Elaeis guineensis Jacq. and Bixa orellana L. and evaluation of its in vitro anticancer activity in human A375 melanoma cells
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DOI：
10.1080/14786419.2017.1320793
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发表时间：
2018-01-01
影响因子：
2.2
通讯作者：
Limonta, Patrizia
中科院分区：
文献类型：
--
作者：
Beretta, Giangiacomo;Gelmini, Fabrizio;Limonta, Patrizia
本研究报道了一种快速、便捷的HPLC-UV-DAD方法，从两种不同的富含维生素E的市售产品中分离出-T3。（油棕）和Bixa orellanaL的种子。(胭脂树树)。色谱柱为反相（RP） C-18，流动相为高效液相色谱级乙腈。经GC-MS和H-1 NMR鉴定，分离得到-T3的纯度在98%以上。与其他不含-T3的工具混合物相比，在体外发现的-T3对人类A375黑色素瘤细胞增殖的细胞毒活性表明，它在实验中对棕榈油衍生产品的抗癌活性中起主要作用。综上所述，这项研究的结果强调了在实验或临床测试之前，应用合适的净化系统来制备生育三烯醇的重要性。
In this work, we report a rapid and convenient HPLC-UV-DAD method for the isolation of -T3 on semi-preparative scale from two different vitamin E rich processed, commercially available products obtained from the fruits of Elaeis guineensis Jacq. (oil palm) and from the seeds of Bixa orellanaL. (achiote tree). Chromatography was run using reverse phase (RP) C-18 columns and HPLC-grade acetonitrile as mobile phase. The purity of the isolated -T3, assessed by GC-MS and H-1 NMR was above 98%. The -T3 cytotoxic activity found in vitro against the proliferation of human A375 melanoma cells compared to that of the other -T3 free tocols mixture suggest its primary role in the experimental anticancer activity observed for palm oil derived products. Taken altogether, the results of this study highlight the importance of the application of suitable purification systems for the preparations of tocotrienols prior to their experimental or clinical testing.[GRAPHICS].