Biological identifications of mayflies (Ephemeroptera) using DNA barcodes
Biological identifications of mayflies (Ephemeroptera) using DNA barcodes
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DOI：
10.1899/04-142.1
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发表时间：
2005-09-01
影响因子：
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通讯作者：
Webb, JM
中科院分区：
文献类型：
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作者：
Ball, SL;Hebert, PDN;Webb, JM
我们测试了DNA条形码在识别主要来自美国东北部和加拿大中部的蜉蝣物种方面的功效。我们对线粒体基因细胞色素c氧化酶1 （COI）的630个碱基对片段进行了测序，从80个物种中每个物种的1个个体中创建了参考序列图谱。我们利用这些参考序列鉴定了70个额外的标本，代表了剖面中的32个物种。DNA条形码，正确识别了70个样本中的69个。唯一的例外是一个形态鉴定为Maccaffertium moderum的个体，它与其他Maccaffertium moderum标本表现出深刻的遗传差异。物种内部的平均序列差异为1%，而同类物种之间的平均差异要大一个数量级（18%）。我们认为DNA条形码技术可以为蜉蝣的种类鉴定提供有力的工具。
We tested the efficacy of DNA barcodes in identifying mayfly species primarily from the northeastern United States and central Canada. We sequenced a 630-base-pair segment of the mitochondrial gene, cytochrome c oxidase 1 (COI), from 1 individual of each of 80 species to create a reference sequence profile. We used these reference sequences to identify 70 additional specimens representing 32 of the species that were in the profile. DNA barcodes, correctly identified 69 of the 70 test specimens. The sole exception was an individual identified morphologically as Maccaffertium modestum that showed deep genetic divergence from other M. modestum specimens. Mean sequence divergence within species was 1%, whereas mean divergence among congeneric species was an order of magnitude greater (18%). We conclude that DNA barcoding can provide a powerful tool for mayfly species identification.