The cytology and cytochemistry of the hemocytes of Mytilus edulis and their responses to experimentally injected carbon particles.
The cytology and cytochemistry of the hemocytes of Mytilus edulis and their responses to experimentally injected carbon particles.
复制标题
DOI：
10.1016/0022-2011(77)90167-7
复制
发表时间：
1977
影响因子：
3.4
通讯作者：
M. Moore;D. Lowe
中科院分区：
文献类型：
--
作者：
M. Moore;D. Lowe
本文对紫贻贝（Mytilusedulis）的血细胞进行了细胞学和细胞化学研究。根据结构、染色反应和吞噬行为，将它们分为两大类：嗜碱性血细胞和嗜酸性颗粒血细胞（粒细胞）。嗜碱性细胞进一步分为小淋巴细胞和较大的吞噬巨噬细胞，对溶酶体水解酶有反应。在粒细胞和小淋巴样细胞中观察到有丝分裂，认为这些细胞是嗜碱性细胞系的干细胞。少数细胞似乎介于淋巴细胞和小粒细胞之间。巨噬细胞是参与注射碳粒清除的主要细胞类型。然而，粒细胞确实显示出一些吞噬活性。棕色细胞表现出明显的变形细胞行为，发现细胞质内含物中含有Fe 3+和Pb 2+，其中一些也与β-葡萄糖醛酸酶和氨基葡萄糖苷酶反应。这些细胞似乎与血细胞有不同的起源。
Hemocytes ofMytilus eduliswere examined cytologically and cytochemically. On the basis of structure, staining reactions, and phagocytic behavior, they were divided into two main groups: basophilic hemocytes and eosinophilic granular hemocytes (granulocytes). The basophilic cells were further divided into small lymphocytes and larger phagocytic macrophages reactive for lysosomal hydrolases. Mitosis was observed in granulocytes and in small lymphoid cells, believed to be the stem cells for the basophilic cell line. A few cells appeared to be intermediate between lymphocytes and small granulocytes. Macrophages were the main cell type involved in the clearance of injected carbon particles. However, granulocytes did show some phagocytic activity. Brown cells displaying apparent amoebocytic behavior were found to contain Fe3+and Pb2+in cytoplasmic inclusions, some of which were also reactive for β-glucuronidase and glucosaminidase. These cells appear to have a separate origin from the hemocytes.