EncephalomyelitisExperimental Autoimmune Protein by Dendritic Cells Leads to Presentation of the Self Antigen Myelin Basic
EncephalomyelitisExperimental Autoimmune Protein by Dendritic Cells Leads to Presentation of the Self Antigen Myelin Basic
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骨髓（BM）来源的树突状细胞（DC）是初始CD 4 1 T细胞活化的有效刺激物。由于DC在Ag加工方面是有效的，并且可能呈递自身Ag，因此我们研究了DC在诱导实验性自身免疫性脑脊髓炎（EAE）中呈递来自髓鞘碱性蛋白（Ac 1-11）的致脑炎肽（encephalitogenic peptide）的作用。为了确定DC是否可以引发EAE，我们将用Ac 1-11或单独用培养基脉冲的DC与从对Ac 1-11 1 I-A u特异性TCR转基因的小鼠分离的CD 4 1 T细胞组合转移到辐射小鼠中。用Ac 1-11脉冲的DC转移的小鼠在7-10天后发生EAE，而接受中等脉冲的DC的小鼠则没有。到第15天，给予负载肽的DC的所有小鼠都有尾部和后肢麻痹的迹象，到第20天，在脑实质中检测到Ac 1-11特异性CD 4 1 T细胞的滤过。我们还证明了Ac 1-11脉冲DC和Ac 1-11特异性T细胞在两种细胞群过继转移后24小时在淋巴结中的相互作用。这些数据表明，DC可以有效地将自身Ag髓鞘碱性蛋白Ac 1-11呈递给小鼠外周中的Ag特异性T细胞，以诱导EAE。免疫学杂志，1999，163：32-39.
Bone marrow (BM)-derived dendritic cells (DC) are potent stimulators of naive CD4 1 T cell activation. Because DC are efficient at Ag processing and could potentially present self Ags, we investigated the role of DC in the presentation of an encephalitogenic peptide from myelin basic protein (Ac 1–11 ) in the induction of experimental autoimmune encephalomyelitis (EAE). To determine if DC could prime for EAE, we transferred DC pulsed with Ac 1–11 or with medium alone into irradiated mice in combination with CD4 1 T cells isolated from a mouse transgenic for a TCR specific for Ac 1–11 1 I-A u . Mice transferred with Ac 1–11 -pulsed DC developed EAE 7–10 days later, whereas mice receiving medium-pulsed DC did not. By day 15, all mice given peptide-loaded DC had signs of tail and hind limb paralysis, and by day 20 infiltration of Ac 1–11 -specific CD4 1 T cells was detected in the brain parenchyma. We also demonstrated interactions between Ac 1–11 -pulsed DC and Ac 1–11 -specific T cells in the lymph nodes 24 h following adoptive transfer of both cell populations. These data show that DC can efficiently present the self Ag myelin basic protein Ac 1–11 to Ag-specific T cells in the periphery of mice to induce EAE. The Journal of Immunology, 1999, 163: 32–39.