New Metabolic Pathway for Converting Blasticidin S in Aspergillus flavus and Inhibitory Activity of Aflatoxin Production by Blasticidin S Metabolites
New Metabolic Pathway for Converting Blasticidin S in Aspergillus flavus and Inhibitory Activity of Aflatoxin Production by Blasticidin S Metabolites
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DOI：
10.1021/jf402745c
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发表时间：
2013-08-21
影响因子：
6.1
通讯作者：
Sugita-Konishi, Yoshiko
中科院分区：
文献类型：
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作者：
Yoshinari, Tomoya;Sakuda, Shohei;Sugita-Konishi, Yoshiko
Blasticidin S是一种蛋白质合成抑制剂，可以抑制黄曲霉毒素的产生而不影响真菌的生长。利用四极杆飞行时间-液相色谱-质谱法对黄曲霉S处理后的代谢产物进行了分析，结果表明，黄曲霉S被代谢为一种新的代谢物n -乙酰脱氨羟基杀虫素S。杀菌素S和n -乙酰杀菌素S对黑曲霉的生长有较强和较弱的抑制作用，而脱胺羟基杀菌素S和n -乙酰去胺羟基杀菌素S对黑曲霉的生长无抑制作用。另一方面，脱胺羟基杀菌素S持续抑制黄曲霉毒素的产生，而n -乙酰脱胺羟基杀菌素S和n -乙酰脱胺羟基杀菌素S则没有。这些结果表明，母泡毒素S和去胺羟基母泡毒素S的C-13处的游离氨基可能对抑制黄曲霉毒素的产生具有重要作用。
Blasticidin S, a protein synthesis inhibitor, inhibits aflatoxin production of Aspergillus flavus without affecting fungal growth. Analysis of metabolites in blasticidin S-treated A. flavus using quadrupole time-of-flight liquid chromatography mass spectrometry showed that blasticidin S was metabolized into a novel metabolite, N-acetyldeaminohydroxyblasticidin S. Conversion of blasticidin S to N-acetyldeaminohydroxyblasticidin S via deaminohydroxyblasticidin S or N-acetylblasticidin S was observed in in vivo and in vitro A. flavus systems. Blasticidin S and N-acetylblasticidin S inhibited the growth of Aspergillus niger strongly and weakly, respectively, but deaminohydroxyblasticidin S and N-acetyldeaminohydroxyblasticidin S did not inhibit its growth. On the other hand, deaminohydroxyblasticidin S sustained the inhibition of aflatoxin production whereas N-acetylblasticidin S and N-acetyldeaminohydroxyblasticidin S did not. These results suggest that the free amino group at C-13 of blasticidin S and deaminohydroxyblasticidin S may be important for the inhibitory activity of aflatoxin production.