Augmentation of human thymocyte proliferative responses by retinoic acid.
Augmentation of human thymocyte proliferative responses by retinoic acid.
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视黄酸增强人胸腺细胞增殖反应。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
S. Golub
中科院分区：
文献类型：
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作者：
N. Sidell;E. Famatiga;S. Golub
当10(-5)至10(-7)M全反式视黄酸(RA)添加到有丝分裂原或同种异体刺激细胞存在的人胸腺细胞或扁桃体淋巴细胞培养物中时，胚细胞发生增加至2.5倍。没有观察到外周血或脾淋巴细胞的增殖反应增强。在胸腺中，不结合花生凝集素 (PNA) 的细胞亚类中的淋巴细胞负责 RA 诱导的增强。 RA 还增加了在软琼脂中发育的有丝分裂原刺激的胸腺细胞集落的数量。这些结果表明，RA 活性的目标必须是比通过与 PNA 结合鉴定的成熟阶段更晚的淋巴细胞，并且 RA 增强的机制之一是经历母细胞转化的淋巴细胞数量的增加。
When 10(-5) to 10(-7) M all-trans retinoic acid (RA) was added to human thymocyte or tonsil lymphocyte cultures in the presence of mitogens or allogeneic stimulator cells, blastogenesis was increased up to 2.5-fold. No augmentation in proliferative responses of peripheral blood or spleen lymphocytes was observed. In the thymus, lymphocytes in the subclass of cells that did not bind peanut agglutinin (PNA) were responsible for the RA-induced enhancement. RA also increased the number of mitogen-stimulated thymocyte colonies developing in soft agar. These results indicate that the targets of RA activity must be lymphoid cells at a later stage of maturation than those identified by binding to PNA, and that one mechanism of RA enhancement is an increase in the number of lymphocytes that undergo blast transformation.