Extended Region of Nodulation Genes in Rhizobium meliloti 1021. I. Phenotypes of Tn5 Insertion Mutants.
Extended Region of Nodulation Genes in Rhizobium meliloti 1021. I. Phenotypes of Tn5 Insertion Mutants.
复制标题
苜蓿根瘤菌 1021 结瘤基因的扩展区域。I. Tn5 插入突变体的表型。
DOI：
10.1093/genetics/117.2.181
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Long,SR
中科院分区：
文献类型：
--
作者：
Swanson,JA;Tu,JK;Ogawa,J;Sanga,R;Fisher,RF;Long,SR
苜蓿根瘤菌Nod突变体WL131是野生型菌株102F51的衍生物，由野生型R的克隆库补充。 meliloti1021 DNA，并回收克隆pRmJT5。 pRmJT5 的转移使苜蓿在其他根瘤菌物种上结瘤，表明 pRmJT5 在确定宿主范围中的作用。 pRmJT5 的诱变揭示了几个片段，其中转座子插入导致结瘤延迟，和/或在宿主苜蓿植物上形成的结瘤数量显着减少。这组突变体表明了 nodgenes 所在的五个区域；一种突变体，nod-216，位于先前未报道的编码结瘤基因的区域。其他突变表型与 nodH、nodFandnodE 的开放阅读框位置以及 2.2-kbEcoRI 片段相关。突变体 innodG 在该菌株中没有改变表型。一种结瘤突变体被证明是commonnodgene 区域的大量缺失。我们提出了一个讨论，比较了对此扩展的 nodgene 区域进行的各种研究。
Rhizobium melilotiNod-mutant WL131, a derivative of wild-type strain 102F51, was complemented by a clone bank of wild-typeR. meliloti1021 DNA, and clone pRmJT5 was recovered. Transfer of pRmJT5 conferred alfalfa nodulation on otherRhizobiumspecies, indicating a role in host range determination for pRmJT5. Mutagenesis of pRmJT5 revealed several segments in which transposon insertion causes delay in nodulation, and/or marked reduction of the number of nodules formed on host alfalfa plants. The set of mutants indicated five regions in whichnodgenes are located; one mutant,nod-216, is located in a region not previously reported to encode a nodulation gene. Other mutant phenotypes correlated with the positions of open reading frames fornodH, nodFandnodE, and with a 2.2-kbEcoRI fragment. A mutant innodGhad no altered phenotype in this strain. One nodulation mutant was shown to be a large deletion of the commonnodgene region. We present a discussion comparing the various studies made on this extendednodgene region.