Quantitative alterations in bovine milk proteome from healthy, subclinical and clinical mastitis during S. aureus infection
Quantitative alterations in bovine milk proteome from healthy, subclinical and clinical mastitis during S. aureus infection
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DOI：
10.1016/j.jprot.2020.103815
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发表时间：
2020-07-15
影响因子：
3.3
通讯作者：
Ambatipudi, Kiran
中科院分区：
文献类型：
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作者：
Maity, Sudipa;Das, Debiprasanna;Ambatipudi, Kiran
由金黄色葡萄球菌引起的牛乳腺炎是产奶的主要障碍，并且缺乏指示奶牛和水牛疾病进展的标记。因此，本研究的重点是鉴定标记从亚临床乳腺炎到临床乳腺炎转变的蛋白质。从 6 组（即健康、亚临床和临床乳腺炎荷斯坦弗里斯兰奶牛和穆拉水牛）中分离出乳清蛋白。对来自奶牛和水牛的 12 个生物样本（每个健康、亚临床和临床乳腺炎样本 4 个）进行了质谱分析和统计分析（方差分析和 t 检验），总共产生了 24 个蛋白质组数据集。总共鉴定了 1479 个蛋白质，其中重要蛋白质通过倍数变化 (= 2) 和 q < 0.05 的组合入围。在这些蛋白质中，128 种和 163 种分别表明牛和水牛的疾病进展。荷斯坦弗里斯兰的触珠蛋白和纤连蛋白以及默拉的精粘素和骨桥蛋白表达的变化与疾病进展相关。同样，在疾病转变过程中，血管生成素和 cofilin-1 上调，而泛素家族成员下调。随后，通过蛋白质印迹验证选定的蛋白质（例如骨桥蛋白和纤维蛋白原-a）。这项研究的结果为乳清蛋白质组动力学和指示疾病进展的特征模式提供了更深入的见解。生物学意义：牛乳腺炎是最致命的传染病，给乳制品行业造成巨大的经济损失。为了了解乳清蛋白组对金黄色葡萄球菌感染的反应动态，我们对从健康、亚临床和临床乳腺炎 HF 和 Mu 中收集的乳清蛋白进行了研究。总共鉴定出 1479 种蛋白质，其中 128 种和 163 种在每个阶段具有指示疾病进展的特征模式。本研究的结果为更好地了解乳腺炎的复杂性奠定了基础，最终将有助于促进早期治疗和基于畜牧业的干预，以改善动物健康和牛奶质量。
Bovine mastitis, caused by Staphylococcus aureus, is a major impediment to milk production and lacks markers to indicate disease progression in cows and buffaloes. Thus, the focus of this study was to identify proteins marking the transition from subclinical to clinical mastitis. Whey proteins were isolated from 6 group's i.e. healthy, subclinical and clinical mastitis of Holstein Friesian cow and Murrah buffalo. Mass spectrometry and statistical analysis (ANOVA and t-tests) were performed on 12 biological samples each from cow and buffalo (4 per healthy, subclinical and clinical mastitis) resulting in a total of 24 proteome datasets. Collectively, 1479 proteins were identified of which significant proteins were shortlisted by a combination of fold change (= 2) and q < 0.05. Of these proteins, 128 and 163 indicated disease progression in cow and buffalo, respectively. Change in expression of haptoglobin and fibronectin from Holstein Friesian while spermadhesin and osteopontin from Murrah correlated with disease progression. Similarly, angiogenin and cofilin-1 were upregulated while ubiquitin family members were downregulated during disease transition. Subsequently, selected proteins (e.g. osteopontin and fibrinogen-a) were validated by Western blots. The results of this study provide deeper insights into whey proteome dynamics and signature patterns indicative of disease progression.Biological significance: Bovine mastitis is the most lethal infectious disease causing a huge economic loss in the dairy industry. In an attempt, to understand the dynamics of whey proteome in response to S. aureus infection, whey protein collected from healthy, subclinical and clinical mastitic HF and Mu were investigated. A total of 1479 proteins were identified, of which 128 and 163 had signature pattern in each stage indicative of the progression of the disease. The results of the present study provide a foundation to better understand the complexity of mastitis that will ultimately help facilitate early therapeutic and husbandry-based intervention to improve animal health and milk quality.