The ATP binding cassette transporter ABC1, is required for the engulfment of corpses generated by apoptotic cell death
The ATP binding cassette transporter ABC1, is required for the engulfment of corpses generated by apoptotic cell death
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DOI：
10.1002/j.1460-2075.1996.tb00353.x
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发表时间：
1996-01-15
期刊：
影响因子：
11.4
通讯作者：
Chimini, G
中科院分区：
文献类型：
--
作者：
Luciani, MF;Chimini, G
ATP结合盒（ABC）转运蛋白定义了一个蛋白质家族，该家族具有在进化过程中保守的强结构相似性，并且致力于多种底物跨细胞膜的易位。该家族的一些成员在哺乳动物中是已知的，但是尽管它们都是医学相关的蛋白质，但对其分子功能的了解仍然很少，本文报道了最近发现的哺乳动物ABC转运蛋白ABC 1的形态学和功能研究。ABC 1在胚胎发育过程中的表达与程序性细胞死亡的区域在时间和空间上相关。更具体地，ABC 1在参与死亡细胞的吞噬和清除的巨噬细胞中表达。此外，吞噬需要ABC 1转运蛋白，因为在抗体介导的ABC 1的空间阻断后，巨噬细胞摄取凋亡小体的能力严重受损。ABC 1的结构同源物已在秀丽隐杆线虫基因组中被鉴定，并定位在ced-7基因座附近。由于ced-7表型由细胞尸体的吞噬受损精确定义，因此很容易推测ABC 1可能是ced-7的哺乳动物同源物。
ATP binding cassette (ABC) transporters define a family of proteins with strong structural similarities conserved across evolution and devoted to the translocation of a variety of substrates across cell membranes, A few members of the family are known in mammals, but although all of them are medically relevant proteins, knowledge of their molecular function remains scanty, We report here a morphological and functional study of the recently identified mammalian ABC transporter, ABC1, Its expression during embryonic development correlates spatially and temporally with the areas of programmed cell death. More specifically, ABC1 is expressed in macrophages engaged in the engulfment and clearance of dead cells, Moreover, ABC1 transporter is required for engulfment since the ability of macrophages to ingest apoptotic bodies is severely impaired after antibody-mediated steric blockade of ABC1. A structural homologue of ABC1 has been identified in the Caenorhabditis elegans genome and maps close to the ced-7 locus, Since ced-7 phenotype is precisely defined by an impaired engulfment of cell corpses, it is tempting to surmise that ABC1 might be a mammalian homologue of ced-7.