INFLUENCE OF LIPOSOME CHARGE ON THE ASSOCIATION OF LIPOSOMES WITH KUPFFER CELLS-INVITRO - EFFECTS OF DIVALENT-CATIONS AND COMPETITION WITH LATEX-PARTICLES
INFLUENCE OF LIPOSOME CHARGE ON THE ASSOCIATION OF LIPOSOMES WITH KUPFFER CELLS-INVITRO - EFFECTS OF DIVALENT-CATIONS AND COMPETITION WITH LATEX-PARTICLES
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DOI：
10.1016/0005-2736(85)90244-5
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
SCHERPHOF, G
中科院分区：
文献类型：
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作者：
DIJKSTRA, J;VANGALEN, M;SCHERPHOF, G
研究了携带不同表面电荷的大单层脂质体与维持培养的大鼠Kupffer细胞的相互作用。除了14c标记的磷脂酰胆碱外，所有脂质体制剂分别含有3h标记的菊粉或125i标记的牛血清白蛋白作为不可降解或可降解的水空间标记。由于囊泡不携带净电荷，内化脂质体的细胞内处理导致蛋白质标签几乎完全以酸溶形式释放到培养基中，而磷脂标签主要被细胞保留，仅约为。1/3被释放。溶酶促缩剂氨的存在抑制了细胞中这两种标记的释放。为4.度。C，囊泡的结合和降解被强烈地减少。这些结果与我们报道的带负电荷脂质体非常相似（Dijkstra et. al., 1984）。两种类型的囊泡的相互作用显然是通过吸附到细胞表面进行的，然后通过内吞作用几乎完全内化。对带正电的囊泡进行的类似实验表明，只有。apprx。脂质体的1/2通过内吞途径被吸收，另一半被吸收到细胞表面。所有类型的脂质体对细胞的附着都强烈依赖于二价阳离子的存在；Ca2+似乎是最佳结合所必需的。中性脂质体与带负电荷的囊泡的摄取仅轻微竞争，均为4度。摄氏37度。C，而带负电荷的小单层囊泡和带负电荷的乳胶珠被发现与带负电荷的大脂质体非常有效地竞争。中性囊泡与带正电的囊泡有效竞争摄取。显然，中性脂质体和带正电的脂质体主要与相同的细胞表面结合位点结合，而带负电的囊泡主要与其他结合位点结合。
The interaction of large unilamellar liposomes carrying different surface charges with rat Kupffer cells in maintenance culture was studied. In addition to 14C-labeled phosphatidylcholine, all liposome preparations contained either 3H-labeled inulin or 125I-labeled bovine serum albumin as a non-degradable or a degradable aqueous space marker, respectively. With vesicles carrying no net charge, intracellular processing of internalized liposomes caused nearly complete release of protein label into the medium in acid-soluble form, while phospholipid label was predominantly retained by the cells, only .apprx. 1/3 being released. The presence of the lysosomotropic agent, ammonia, inhibited the release of both labels from the cells. At 4.degree. C, the association and degradation of the vesicles were strongly reduced. These results are very similar to what we reported on negatively charged liposomes (Dijkstra et. al., 1984). The interaction of both types of vesicles apparently proceeds by adsorption to the cell surface followed by virtually complete internalization by endocytosis. Similar experiments with positively charged vesicles indicated that only .apprx. 1/2 of the liposomes were taken up by the endocytic route, the other half remaining absorbed to the cell-surface. Attachment of all types of liposomes to the cells was strongly dependent on the presence of divalent cations; Ca2+ appeared to be required for optimal binding. Neutral liposomes only slightly competed with the uptake of negatively charged vesicles, both at 4.degree. C and 37.degree. C, whereas negatively charged small unilamellar vesicles and negatively charged latex beads were found to compete very effectively with the large negatively charged liposomes. Neutral vesicles competed effectively for uptake with positively charged ones. Apparently, neutral and positively charged liposomes are largely bound by the same cell-surface binding sites, while negatively charged vesicles attach mainly to other binding sites.