A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish
A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish
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DOI：
10.1016/j.neuroscience.2006.12.073
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发表时间：
2007-05-25
期刊：
影响因子：
3.3
通讯作者：
Guo, S.
中科院分区：
文献类型：
--
作者：
Bretaud, S.;Li, Q.;Guo, S.
透明的斑马鱼幼鱼提供了一个机会，解开遗传和神经网络的基础行为在一个发展中的系统。在这项研究中，我们开发了一个选择室范式来衡量奖励相关的行为在斑马鱼幼虫。在斑马鱼幼虫可以选择在含有水或吗啡的隔室中度过时间的室中，先前经历过吗啡的幼虫在含有吗啡的隔室中度过的时间明显更多。这种行为可以通过用阿片受体或多巴胺受体的拮抗剂预处理来减弱，并且此外，在太少的突变体中受损，所述突变体在腹侧前脑中产生特定组的多巴胺能和多巴胺能神经元中具有遗传缺陷。这些结果揭示了一个选择行为的成瘾物质在斑马鱼幼虫，是通过中央阿片和单胺能神经递质系统介导的。(c)2006由Elsevier Ltd代表IBRO出版。
Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their time in either a water-or morphine-containing compartment, larvae that have previously experienced morphine spend significantly more time in the compartment containing morphine. This behavior can be attentuated by pre-treatment with antagonists of the opioid receptor or the dopamine receptor, and furthermore, is impaired in the too few mutant, which has a genetic deficiency in the production of specific groups of dopaminergic and serotonergic neurons in the ventral forebrain. These results uncover a choice behavior for an addictive substance in larval zebrafish that is mediated through central opioid and monoaminergic neurotransmitter systems. (c) 2006 Published by Elsevier Ltd on behalf of IBRO.