Monoclonal antibodies against leishmanial membranes react with specific excreted factors (EF).
Monoclonal antibodies against leishmanial membranes react with specific excreted factors (EF).
复制标题
抗利什曼原虫膜的单克隆抗体与特定的分泌因子 (EF) 发生反应。
DOI：
10.1080/00034983.1990.11812494
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发表时间：
1990
影响因子：
--
通讯作者：
Jaffe,CL
中科院分区：
文献类型：
--
作者：
Schnur,LF;Sarfstein,R;Jaffe,CL
The interaction was examined between two reagents used for the speciation ofLeishmania: spent culturemedium excreted factors (EF) and antileishmanial monoclonal antibodies (MCA). Thirty-three MCAs: seven againstL. major, five againstL. tropica, eight againstL aethiopica, 11 againstL. donovani sensu latoand two against allLeishmaniaspecies were screened by double diffusion for reactions with EFs representing seven different sub-serotypes (A1, A2, A4B1, B2, B3and A3B2). Only five MCAs showed any reactivity. Three MCAs toL. major(T1, T2 and T7) precipitated only sub-serotype A1and A4EF, and two MCAs toL. tropica(T11 and T15) precipitated only sub-serotype A2EF.Leishmania majorandL. tropicaare both serotype A species. None of the antiL.aethiopicaand antiL.donovaniMCAs reacted with any of the EF preparations. Both of these species belong to the serotype B. MCAs T11 and T15, the first recorded with a specificity for only sub-serotype A2EF, were tested further against 28 sub-serotype A2and three sub-serotype A2B2EFs fromL. tropicastrains. All 31 EFs formed visible bands with standard rabbit polyclonal antileishmanial serotype A serum. Only 18 of them precipitated with T11 and T15, even though parasite lysates prepared from promastigotes of several non-reactors tested positive with the MCAs. The lack of homogenous reactivity with T11 and T15 among the A2EFs suggests that the EFs from these strains possess structural differences which affect EF-MCA precipitation.
