Interaction of bluetongue virus with bovine lymphocytes.
Interaction of bluetongue virus with bovine lymphocytes.
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蓝舌病毒与牛淋巴细胞的相互作用。
DOI：
10.1099/0022-1317-71-2-363
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Stott,ML
中科院分区：
文献类型：
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作者：
Stott,JL;Blanchard-Channell,M;Scibienski,RJ;Stott,ML
新鲜分离并建立的牛外周血单核白细胞（PBML）培养物暴露于蓝舌病毒（BTV），以确定潜在的亲淋巴细胞性。在存在白细胞介素2（IL-2）和有丝分裂原的情况下建立PBML培养物，并在感染性研究前作为批量培养物或克隆进行维持。基于以下特征，所有培养物似乎都是T细胞表型：T淋巴细胞特异性凝集素（即花生凝集素和苹果凝集素）的结合，绵羊红细胞的玫瑰花结，推定的泛T单克隆抗体的结合，以及表面免疫球蛋白（IG）的缺乏。T淋巴细胞培养物的特征还在于其引起凝集素依赖性细胞毒性（LDCC）的能力。建立淋巴细胞培养暴露于BTV导致生产性细胞病变和非细胞病变感染。在LDCC阴性培养物中观察到非致细胞病变的生产性感染，而在LDCC阳性培养物中观察到致细胞病变和非致细胞病变的感染。新鲜分离的PBML暴露于BTV导致最小的病毒复制;向这样的培养物中加入促分裂原和IL-2并不增加病毒复制。添加促分裂原和IL- 2诱导的原始细胞转化可忽略不计，而PBML活力受到的影响最小。这些研究建立了BTV对牛T淋巴细胞的嗜性，病毒复制仅限于那些进行胚细胞发生的细胞。建立感染的淋巴细胞培养物，而不丧失培养物活力，表明这种相互作用可能导致与牛BTV感染相关的长期病毒血症。
Freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (PBMLs) were exposed to bluetongue virus (BTV) for the purpose of defining potential lymphotropism. PBML cultures were established in the presence of interleukin 2 (IL-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. All cultures appeared to be of the T cell phenotype based on the following characteristics: binding of T lymphocyte-specific lectins (i.e. peanut agglutinin andHelix pomatia), rosetting of sheep erythrocytes, binding of a putative pan-T monoclonal antibody, and absence of surface immunoglobulin (Ig). T lymphocyte cultures were further characterized by their ability to elicit lectin-dependent cellular cytotoxicity (LDCC). Exposure of established lymphocyte cultures to BTV resulted in productive cytopathic and non-cytopathic infections. Non-cytopathic productive infections were observed in LDCC-negative cultures whereas cytopathic and non-cytopathic infections were observed in LDCC-positive cultures. Exposure of freshly isolated PBMLs to BTV resulted in minimal virus replication; addition of mitogen and IL-2 to such cultures did not augment virus replication. Addition of mitogen and IL- 2 induced negligible blast transformation, whereas PBML viability was minimally affected. These studies establish a tropism of BTV for bovine T lymphocytes with virus replication being limited to those cells undergoing blastogenesis. Establishment of infected lymphocyte cultures, without loss of culture viability, suggest such an interaction may contribute to the long term viraemias associated with BTV infection of cattle.