A NEW ORPHAN MEMBER OF THE NUCLEAR HORMONE-RECEPTOR SUPERFAMILY THAT INTERACTS WITH A SUBSET OF RETINOIC ACID RESPONSE ELEMENTS
A NEW ORPHAN MEMBER OF THE NUCLEAR HORMONE-RECEPTOR SUPERFAMILY THAT INTERACTS WITH A SUBSET OF RETINOIC ACID RESPONSE ELEMENTS
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DOI：
10.1128/mcb.14.3.1544
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发表时间：
1994-03-01
影响因子：
5.3
通讯作者：
MOORE, DD
中科院分区：
文献类型：
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作者：
BAES, M;GULICK, T;MOORE, DD
我们已经确定并描述了核激素受体超家族的一个新的孤儿成员，称为MB67，主要在肝脏中表达。MB67结合并激活维甲酸反应元件，控制维甲酸受体β 2和乙醇脱氢酶3基因的表达，这两个基因都由与共识AGGTCA相关的直接重复六聚体组成，间隔5bp。MB67将这些元素作为异源二聚体与9-顺式维甲酸受体RXR结合。然而，MB67不结合或激活其他具有替代六聚体排列的维甲酸反应元件或其他几种野生型和合成激素反应元件。MB67对维甲酸反应元件的反式激活比维甲酸受体所赋予的反应元件弱，但不需要全反式维甲酸、9-顺式维甲酸或任何外源添加的配体的存在。我们提出MB67在控制对维甲酸及其代谢物反应的复杂蛋白质网络中起重要作用。
We have identified and characterized a new orphan member of the nuclear hormone receptor superfamily, called MB67, which is predominantly expressed in liver. MB67 binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes, both of which consist of a direct repeat hexamers related to the consensus AGGTCA, separated by 5 bp. MB67 binds these elements as a heterodimer with the 9-cis-retinoic acid receptor, RXR. However, MB67 does not bind or activate other retinoic acid response elements with alternative hexamer arrangements or any of several other wild-type and synthetic hormone response elements examined. The transactivation of retinoic acid response elements by MB67 is weaker than that conferred by the retinoic acid receptors but does not require the presence of all-trans retinoic acid, 9-cis-retinoic acid, or any exogenously added ligand, We propose that MB67 plays an important role in the complex network of proteins that govern response to retinoic acid and its metabolites.