Polymer-coated nanoparticles: a universal tool for biolabelling experiments.
Polymer-coated nanoparticles: a universal tool for biolabelling experiments.
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DOI：
10.1002/smll.201100608
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发表时间：
2011-11
期刊：
影响因子：
13.3
通讯作者：
Feng Zhang;Emma E. Lees;F. Amin;Pilar Rivera Gil;Fang Yang;P. Mulvaney;W. Parak
中科院分区：
文献类型：
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作者：
Feng Zhang;Emma E. Lees;F. Amin;Pilar Rivera Gil;Fang Yang;P. Mulvaney;W. Parak
纳米颗粒的水溶性是许多生物应用的基本先决条件。迄今为止，没有一种方法是理想的，已经成功地利用了几种不同的方法。这些“相转移”的策略进行审查，指出主要的优点和缺点，并提出了共轭策略的讨论。用两亲聚合物涂覆疏水纳米颗粒提供了半导体、磁性、金属和上转换纳米颗粒从非极性环境到极性环境的相转移的通用途径。包括马来酰亚胺基团的两亲性聚合物可以容易地用化学基团官能化以用于特定应用。在第二，实验部分，这种聚合物封端的纳米粒子的一些新的化学特性进行了演示。特别是，纳米粒子的pH敏感的荧光团已被连接的描述，并证明其用于细胞内pH传感。结果表明，分析物敏感的荧光团的性质可以通过使用与底层纳米颗粒的相互作用来调节。
Water solubilization of nanoparticles is a fundamental prerequisite for many biological applications. To date, no single method has emerged as ideal, and several different approaches have been successfully utilized. These 'phase-transfer' strategies are reviewed, indicating key advantages and disadvantages, and a discussion of conjugation strategies is presented. Coating of hydrophobic nanoparticles with amphiphilic polymers provides a generic pathway for the phase transfer of semiconductor, magnetic, metallic, and upconverting nanoparticles from nonpolar to polar environments. Amphiphilic polymers that include maleimide groups can be readily functionalized with chemical groups for specific applications. In the second, experimental part, some of the new chemical features of such polymer-capped nanoparticles are demonstrated. In particular, nanoparticles to which a pH sensitive fluorophore has been attached are described, and their use for intracellular pH-sensing demonstrated. It is shown that the properties of analyte-sensitive fluorophores can be tuned by using interactions with the underlying nanoparticles.