Biologically active protein nanoarrays generated using parallel dip-pen nanolithography
Biologically active protein nanoarrays generated using parallel dip-pen nanolithography
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DOI：
10.1002/adma.200600070
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发表时间：
2006-05-02
影响因子：
29.4
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Lee, Seung Woo;Oh, Byung-Keun;Mirkin, Chad A.
采用平行浸笔纳米光刻技术（DPN）制备了氨基活性n -羟基琥珀酰亚胺端烷基硫醇模板，并用于蛋白A/G共价偶联。生成的蛋白质阵列（见图）用于通过亲和结合捕获抗体，同时保留其生物识别特性。描述了并行DPN方法的通用性，用于以相对高通量的方式（10分钟内14000个点）制造许多类似的结构。
Amine-active N-hydroxysuccinimide-terminated alkyl thiol templates are generated using parallel dip-pen nanolithography (DPN) and are used to covalently couple protein A/G. The protein arrays generated (see figure) are used to capture antibodies through affinity binding, while preserving their biological recognition properties. The versatility of the parallel DPN method for making many similar structures in a relatively high-throughput manner (14 000 dots in 10 min) is described.