An efficient use of the WATERGATE W5 sequence for observing a ligand binding with a protein receptor
An efficient use of the WATERGATE W5 sequence for observing a ligand binding with a protein receptor
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DOI：
10.1002/mrc.2264
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发表时间：
2008-09-01
影响因子：
2
通讯作者：
Tashiro, Mitsuru
中科院分区：
文献类型：
--
作者：
Furihata, Kazuo;Shimotakahara, Sakurako;Tashiro, Mitsuru
通过引入WATERGATE W5序列，已经开发了用于观察配体与受体结合的有效脉冲序列。在通过梯度光谱（WaterLOGSY）技术观察的常规水配体中，在脉冲序列的初始部分使用例如双脉冲场梯度自旋回波（DPFGSE）序列选择性地激发水共振。在当前版本中，修改的WATERGATE W5序列被合并在脉冲序列的初始部分，并且水频率处的谐振可以通过修改的WATERGATE W5序列选择性地保留。已证明使用人血清白蛋白（HSA）-色氨酸复合物的配体观察的NMR筛选技术的效率。版权所有（c）2008约翰威利父子有限公司。
An efficient pulse sequence for observing a ligand binding with a receptor has been developed by incorporating the WATERGATE W5 sequence. In the conventional water ligand observed via gradient spectroscopy (WaterLOGSY) techniques, the water resonance is selectively excited using,e.g. the double-pulsed field gradient spin-echo (DPFGSE) sequence at the initial portion of pulse sequence. In the current version, the modified WATERGATE W5 sequence is incorporated at the initial portion of the pulse sequence, and the resonance at the water frequency can be selectively reserved by the modified WATERGATE W5 sequence. The efficiency of ligand-observed NMR screening techniques has been demonstrated using the human serum albumin (HSA)-tryptophan complex. Copyright (c) 2008 John Wiley & Sons, Ltd.