MUTATIONS IN A TEMPERATE BACTERIOPHAGE AFFECTING ITS ABILITY TO LYSOGENIZE ESCHERICHIA-COLI
MUTATIONS IN A TEMPERATE BACTERIOPHAGE AFFECTING ITS ABILITY TO LYSOGENIZE ESCHERICHIA-COLI
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DOI：
10.1016/0042-6822(57)90022-3
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发表时间：
1957-01-01
期刊：
影响因子：
3.7
通讯作者：
KAISER, AD
中科院分区：
文献类型：
--
作者：
KAISER, AD
分离出四十个独立发生的清晰噬斑形成的γ突变体。其中可以识别3种不同的表型。突变体之间的杂交表明所有突变位点彼此紧密连锁，占据γ连锁群的一段，其约为已知总遗传长度的1/10。更详细的绘图表明，突变位点落入三个相邻区域，每个表型对应一个区域。一对表型不同的突变体的混合感染，每个突变体的溶原作用都很差或根本没有，产生野生型感染的高频率溶原作用特征。这种现象称为合作。在这种混合感染中幸存的细菌对于一种或两种感染类型的噬菌体是溶原性的。具有相同表型的两个非等位基因明确突变在溶原化中表现出顺反位效应。合作动力学表明溶原化中存在中间状态。
Forty independently occurring clear plaque-forming mutants of [gamma] were isolated. Among them 3 different phenotypes can be recognized. Crosses between mutants indicate that all of the mutant loci are closely linked to one another, occupying a segment of the [gamma] linkage group which is about 1/10 of the total known genetic length. More detailed mapping reveals that the mutant loci fall into three adjacent regions, one for each phenotype. Mixed infection with a pair of phenotypically different mutants, each of which lysogenizes poorly or not at all, produces the high frequency of lysogenization characteristic of infection with wild type. This phenomenon is called cooperation. The bacteria surviving such a mixed infection are lysogenic either for one or both of the infecting types of phages. Two non-allelic clear mutations having the same phenotype show a cis -trans position effect in lysogenization. The kinetics of cooperation suggest the existence of an intermediate state in lysogenization.