Extension of flower longevity in transgenic torenia plants incorporating ACC oxidase transgene
Extension of flower longevity in transgenic torenia plants incorporating ACC oxidase transgene
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DOI：
10.1016/s0168-9452(98)00139-3
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发表时间：
1998-11-02
期刊：
影响因子：
5.2
通讯作者：
Shibata, M
中科院分区：
文献类型：
--
作者：
Aida, R;Yoshida, T;Shibata, M
我们通过以有义或反义方向引入 1-氨基环丙烷-1-羧酸 (ACC) 氧化酶基因片段，证明了蓝猪草 (Torenia fournieri Lind.) 花寿命的延长。通过农杆菌介导的基因转移转化蓝猪耳。在初级转化体中，8株引入有义基因的植物（平均花寿命2.7-7.1天）和3株引入反义基因的植物（Dom 2.5-2.7天）显示出比野生型植物（2.0天）显着更长的寿命。对后代的分析表明，引入的基因已经遗传，花寿命的延长与该基因的存在有关。 Northern 印迹分析和乙烯产量测量表明，植物中 mRNA 水平和内源乙烯产量降低，花寿命延长。 (C) 1998 Elsevier Science Ireland Ltd. 保留所有权利。
We demonstrated extension of flower longevity in torenia (Torenia fournieri Lind.) by introducing a fragment of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene in sense or antisense orientation. Torenia was transformed by Agrobacterium-mediated gene transfer. Among primary transformants, 8 sense-gene-introduced plants (average flower longevity 2.7-7.1 days) and 3 antisense-gene-introduced plants (Dom 2.5-2.7 days) showed significantly greater longevity than did wild-type plants (2.0 days). Analysis of offspring suggested that the introduced gene had been inherited, and that the extended flower longevity was linked to the existence of the gene. Northern blot analysis and measurement of ethylene production demonstrated a reduction in mRNA level and endogenous ethylene production in plants with improved flower longevity. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.